Recombinant Human R-spondin-1 (RSPO1), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-Avi-tagged, 30.2kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human R-spondin-1 (RSPO1), partial (Active)

Recombinant Human R-spondin-1 (RSPO1), partial (Active) — Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Purity >95%.

SKU: BCRECP-001569 Categories: ,
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Product Specifications

Product SkuBCRECP-001569
Product DescriptionRecombinant Human R-spondin-1 (RSPO1) Protein is expressed from Mammalian cell with C-terminal 10xHis-Avi-tagged. It contains 31-263aa. [Accession | Q2MKA7].
Uniprot No.Q2MKA7
Gene NamesRSPO1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region31-263aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-Avi-tagged
Molecular weight30.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasDevelopmental Biology

Function

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.

Biological Context

Subcellular Location: Secreted; Nucleus
Tissue Specificity: Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen
Disease Association: Keratoderma, palmoplantar, with squamous cell carcinoma of skin and sex reversal (PKKSCC) : A recessive syndrome characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human R-spondin-1 (RSPO1), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 31-263aa, with C-terminal 10xHis-Avi-tagged tag, molecular weight 30.2kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed:29769720). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (PubMed:17804805)

Subcellular Location

Secreted; Nucleus

Disease Association

Keratoderma, palmoplantar, with squamous cell carcinoma of skin and sex reversal (PKKSCC) : A recessive syndrome characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen

Subunit

Interacts with the extracellular domain of FZD8 and LRP6. It however does not form a ternary complex with FZD8 and LRP6. Interacts with WNT1. Binds heparin (By similarity). Interacts with ZNRF3; promoting indirect interaction between ZNRF3 and LGR4 and membrane clearance of ZNRF3. Interacts with LGR4, LGR5 and LGR6. Identified in a complex composed of RNF43, LGR5 and RSPO1. Interacts (via FU repeats) with KREM1 (PubMed:17804805)

Gene: RSPO1  |  Organism: Homo sapiens  |  Synonyms: Roof plate-specific spondin-1
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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