Recombinant Human Ras-related protein Rab-11A (RAB11A), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 39.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Ras-related protein Rab-11A (RAB11A)

Recombinant Human Ras-related protein Rab-11A (RAB11A) — The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Purity >90%.

SKU: BCRECP-001502 Category:
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Product Specifications

Product SkuBCRECP-001502
Product DescriptionRecombinant Human Ras-related protein Rab-11A (RAB11A) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 2-213aa. [Accession | P62491].
Uniprot No.P62491
Gene NamesRAB11A
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-213aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight39.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB11A regulates endocytic recycling.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor); Endosome membrane (Lipid-anchor); Recycling endosome membrane (Lipid-anchor); Cleavage furrow; Cytoplasmic vesicle, phagosome membrane (Lipid-anchor); Golgi apparatus membrane (Lipid-anchor); Golgi apparatus, trans-Golgi network; Cytoplasmic vesicle membrane (Lipid-anchor); Cell projection, cilium

Product Specifications

Recombinant Human Ras-related protein Rab-11A (RAB11A) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-213aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 39.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:15601896, PubMed:15689490, PubMed:17462998, PubMed:19542231, PubMed:20026645, PubMed:20890297, PubMed:21282656, PubMed:26032412). RAB11A regulates endocytic recycling (PubMed:20026645). Forms a functional Rab11/RAB11FIP3/dynein complex that regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endosomal recycling compartment (ERC) (PubMed:20026645). Acts as a major regulator of membrane delivery during cytokinesis (PubMed:15601896). Together with MYO5B and RAB8A participates in epithelial cell polarization (PubMed:21282656). Together with Rabin8/RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells (PubMed:17462998). Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane (PubMed:19542231). Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane (PubMed:15689490). Regulates the recycling of FCGRT (receptor of Fc region of monomeric IgG) to basolateral membranes (By similarity). May also play a role in melanosome transport and release from melanocytes (By similarity). Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation (PubMed:25673879, PubMed:31204173). On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth (PubMed:31204173). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-endososomal dependent export route via interaction with WDR44 (PubMed:32344433). Also interacts with RABL3 to promote ciliary vesicle formation (PubMed:36052645)

Subcellular Location

Cell membrane (Lipid-anchor); Endosome membrane (Lipid-anchor); Recycling endosome membrane (Lipid-anchor); Cleavage furrow; Cytoplasmic vesicle, phagosome membrane (Lipid-anchor); Golgi apparatus membrane (Lipid-anchor); Golgi apparatus, trans-Golgi network; Cytoplasmic vesicle membrane (Lipid-anchor); Cell projection, cilium

Subunit

Interacts (GTP-bound form) with RAB11FIPs (via their C-termini) including RAB11FIP1, RAB11FIP2, RAB11FIP3, RAB11FIP4 and RAB11FIP5 effectors (PubMed:11495908, PubMed:11786538, PubMed:12470645, PubMed:15173169, PubMed:15181150, PubMed:15280022, PubMed:15601896, PubMed:16148947, PubMed:16905101, PubMed:17030804, PubMed:17229837, PubMed:20026645, PubMed:25673879, PubMed:26032412, PubMed:26258637, PubMed:31204173). Interacts with RAB11FIP5 and STXBP6. Interacts with SGSM1, SGSM2 and SGSM3 (By similarity). Interacts with EXOC6 in a GTP-dependent manner (By similarity). Forms a complex with RAB11FIP3 and dynein intermediate chain DYNC1LI1; the interaction between RAB11A1 and RAB11FIP3 is direct; the complex regulates endocytic trafficking (PubMed:20026645). Interacts with EVI5; EVI5 and RAB11FIP3 may be mutually exclusive and compete for binding RAB11A (By similarity). Interacts with STXBP6 (By similarity). Interacts with VIPAS39. Interacts with MYO5B. Found in a complex with MYO5B and CFTR. Interacts with NPC1L1. Interacts (GDP-bound form) with ZFYVE27 (PubMed:17082457, PubMed:21976701). Interacts with BIRC6/bruce. May interact with TBC1D14. Interacts with UNC119; in a cell cycle-dependent manner. GDP-bound and nucleotide-free forms interact with SH3BP5 (PubMed:26506309, PubMed:30217979). Interacts (GDP-bound form) with KIF5A in a ZFYVE27-dependent manner (PubMed:21976701). Interacts (GDP-bound form) with RELCH (By similarity). Found in a complex composed of RELCH, OSBP1 and RAB11A (By similarity). Interacts with TBC1D12 (PubMed:28384198). Interacts with DEF6 (PubMed:31308374). Interacts with VPS33B (PubMed:28017832). Interacts with ATP9A (PubMed:36604604). Forms a heterotetramer with RAB11FIP3; the GTP-bound form is preferred for binding (PubMed:26258637). Forms a complex with Rabin8/RAB3IP and RAB11FIP3, probably a heterohexamer with two of each protein subunit, where Rabin8/RAB3IP and RAB11FIP3 simultaneously bind to RAB11A; the complex promotes preciliary trafficking and cilia growth (PubMed:26258637, PubMed:31204173). Forms a complex containing RAB11A, ASAP1, Rabin8/RAB3IP, RAP11FIP3 and ARF4; the complex promotes preciliary trafficking; the complex binds to RHO in photoreceptor cells and promotes RHO ciliary transport (PubMed:25673879, PubMed:26258637). Interacts (GTP-bound form) with WDR44; the interaction prevents RAB11A-RAB3IP-RAB11FIP3 complex formation (PubMed:10077598, PubMed:31204173, PubMed:32344433). Interacts with SH3TC2 (PubMed:20028792). Interacts (GTP-bound) with RABL3 (GDP-bound); the interaction stabilizes RABL3 and regulates ciliary vesicle formation during early ciliogenesis (PubMed:36052645)

Gene: RAB11A  |  Organism: Homo sapiens  |  Synonyms: YL8
Key Publications

Frequently Asked Questions

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Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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