Recombinant Human Receptor-type tyrosine-protein kinase FLT3 (FLT3), partial, Biotinylated (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-Avi-tagged, 63.2 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Receptor-type tyrosine-protein kinase FLT3 (FLT3), partial, Biotinylated (Active)

Recombinant Human Receptor-type tyrosine-protein kinase FLT3 (FLT3), partial, Biotinylated (Active) — Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic c…

SKU: BCACP-000639 Categories: , , , ,
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Product SKUBCACP-000639
Product DescriptionRecombinant Human Receptor-type tyrosine-protein kinase FLT3 (FLT3), Biotinylated protein is expressed from Mammalian cell with C-terminal 10xHis-Avi-tagged. It contains 27-543aa. [Accession | P36888].
Uniprot No.P36888
Gene NamesFLT3
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region27-543aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-Avi-tagged
Molecular weight63.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human KIR2DL3 at 2 μg/mL can bind Anti-KIR2DL3 recombinant antibody . The EC50 is 7.203-8.603 ng/mL.;KIR2DL3 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human KIR2DL3 with an affinity constant of 0.67 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesReceptor-type tyrosine-protein kinase FLT3; EC 2.7.10.1; FL cytokine receptor; Fetal liver kinase-2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; CD antigen CD135

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.

Biological Context

Subcellular Location: Membrane (Single-pass type I membrane protein); Endoplasmic reticulum lumen
Tissue Specificity: Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia
Disease Association: Leukemia, acute myelogenous (AML) : A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes. [The gene represented in this entry may be involved in disease pathogenesis. Somatic mutations that lead to constitutive activation of FLT3 are frequent in AML patients. These mutations fall into two classes, the most common being in-frame internal tandem duplications of variable length in the juxtamembrane region that disrupt the normal regulation of the kinase activity. Likewise, point mutations in the activation loop of the kinase domain can result in a constitutively activated kinase]

Product Specifications

Recombinant Human Receptor-type tyrosine-protein kinase FLT3 (FLT3), partial, Biotinylated (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 27-543aa, with C-terminal 10xHis-Avi-tagged tag, molecular weight 63.2 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human KIR2DL3 at 2 μg/mL can bind Anti-KIR2DL3 recombinant antibody . The EC50 is 7.203-8.603 ng/mL.;KIR2DL3 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human KIR2DL3 with an affinity constant of 0.67 nM as detected by MetaSPR Assay (WeSPRTM 200).

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways

Subcellular Location

Membrane (Single-pass type I membrane protein); Endoplasmic reticulum lumen

Disease Association

Leukemia, acute myelogenous (AML) : A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes. [The gene represented in this entry may be involved in disease pathogenesis. Somatic mutations that lead to constitutive activation of FLT3 are frequent in AML patients. These mutations fall into two classes, the most common being in-frame internal tandem duplications of variable length in the juxtamembrane region that disrupt the normal regulation of the kinase activity. Likewise, point mutations in the activation loop of the kinase domain can result in a constitutively activated kinase]

Tissue Specificity

Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia

Subunit

(Microbial infection) Interacts with human cytomegalovirus protein UL7

Gene: FLT3  |  Organism: Homo sapiens  |  Synonyms: FL cytokine receptor; Fetal liver kinase-2; Fms-like tyrosine kinase 3; Stem cell tyrosine kinase 1
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