Recombinant Human Rho guanine nucleotide exchange factor 12 (ARHGEF12), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 28.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Rho guanine nucleotide exchange factor 12 (ARHGEF12), partial

Recombinant Human Rho guanine nucleotide exchange factor 12 (ARHGEF12), partial — Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Purity >90%.

SKU: BCRECP-000122 Category:
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Product Specifications

Product SkuBCRECP-000122
Product DescriptionRecombinant Human Rho guanine nucleotide exchange factor 12 (ARHGEF12) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 367-558aa. [Accession | Q9NZN5].
Uniprot No.Q9NZN5
Gene NamesARHGEF12
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region367-558aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight28.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Promotes endothelial cell and actin stress fiber reorientation in response to mechanotransduction.

Biological Context

Subcellular Location: Cytoplasm; Membrane
Tissue Specificity: Ubiquitously expressed. Isoform 2 is found in jejunum and testis
Disease Association: [A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with KMT2A/MLL1]

Product Specifications

Recombinant Human Rho guanine nucleotide exchange factor 12 (ARHGEF12), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 367-558aa, with N-terminal 10xHis-tagged tag, molecular weight 28.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13 (PubMed:11094164). May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) (PubMed:11094164). Promotes endothelial cell and actin stress fiber reorientation in response to mechanotransduction (By similarity)

Subcellular Location

Cytoplasm; Membrane

Disease Association

[A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with KMT2A/MLL1]

Tissue Specificity

Ubiquitously expressed. Isoform 2 is found in jejunum and testis

Subunit

Interacts (probably via RGSL domain) with GNA12 and GNA13 (PubMed:11094164). Interacts with RHOA (PubMed:11094164, PubMed:15331592). Interacts with PLXNB1 (PubMed:12183458, PubMed:12372594, PubMed:18411422). Interacts with PLXNB2 (PubMed:12183458, PubMed:12372594). Interacts (via PDZ domain) with IGF1R beta subunit (PubMed:11724822). Interacts with GCSAM (PubMed:20844236). Interacts with PLEKHG4B; the interaction releases PLEKHG4B autoinhibition and results in activation of CDC42 (PubMed:32203420)

Gene: ARHGEF12  |  Organism: Homo sapiens  |  Synonyms: Leukemia-associated RhoGEF
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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