Recombinant Human Rho-related GTP-binding protein RhoD (RHOD) — Involved in endosome dynamics. Purity >90%.
| Product Sku | BCRECP-001535 |
| Product Description | Recombinant Human Rho-related GTP-binding protein RhoD (RHOD) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-207aa. [Accession | O00212]. |
| Uniprot No. | O00212 |
| Gene Names | RHOD |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 1-207aa |
| Species | Homo sapiens (Human) |
| Tag Info | N-terminal 10xHis-tagged and C-terminal Myc-tagged |
| Molecular weight | 30.6kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Signal Transduction |
Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton.
Recombinant Human Rho-related GTP-binding protein RhoD (RHOD) — Involved in endosome dynamics. Purity >90%. Suitable for ELISA and Western Blot applications.
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution
Cell membrane (Lipid-anchor); Early endosome
Heart, placenta, liver, skeletal muscle, and pancreas and, with weaker intensity, in several other tissues
Interacts (in GTP-bound form) with DIAPH2 isoform 3 (PubMed:12577064). Interacts (in GTP-bound form) with DAPK3 (PubMed:23454120). Interacts (in GTP-bound form) with FILIP1 (PubMed:12577064). Interacts (in GTP-bound form) with WHAMM (By similarity). Interacts with PAK5 (PubMed:17064668). Interacts (independent of GTP-loaded status) with ANKFY1 (PubMed:24102721). Interacts with PLXNB2 (PubMed:34114565)
Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.
Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
