Recombinant Human Semaphorin-4D (SEMA4D), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 81.9 kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Semaphorin-4D (SEMA4D), partial (Active)

Recombinant Human Semaphorin-4D (SEMA4D), partial (Active) — Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Purity >90%.

SKU: BCACP-000120 Category:
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Product SKUBCACP-000120
Product DescriptionRecombinant Human Semaphorin-4D (SEMA4D) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 22-734aa. [Accession | Q92854].
Uniprot No.Q92854
Gene NamesSEMA4D
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region22-734aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight81.9 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human TNFSF11 at 2 μg/mL can bind Human TNFRSF11B . The EC50 is 4.514-5.152 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(A8)(BB18)(GR3)(CD100)

Function

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver

Product Specifications

Recombinant Human Semaphorin-4D (SEMA4D), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 22-734aa, with C-terminal 10xHis-tagged tag, molecular weight 81.9 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed:20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed:19788569). Promotes the migration of cerebellar granule cells (PubMed:16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed:8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed:16055703)

Subcellular Location

Secreted

Tissue Specificity

Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver

Subunit

Homodimer (PubMed:20877282). Interacts with PLXNB2 (By similarity). Interacts with PLXNB1 (PubMed:10520995)

Gene: SEMA4D  |  Organism: Homo sapiens  |  Synonyms: A8; BB18; GR3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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