Recombinant Human Serine/arginine-rich splicing factor 3 (SRSF3), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-GB1-tagged, 17.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Serine/arginine-rich splicing factor 3 (SRSF3), partial

Recombinant Human Serine/arginine-rich splicing factor 3 (SRSF3), partial — Splicing factor, which binds the consensus motif 5′-C[ACU][AU]C[ACU][AC]C-3′ within pre-mRNA and promotes specific exons inclusion during alternative splicing. Purity >90%.

SKU: BCRECP-001697 Category:
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Product Specifications

Product SkuBCRECP-001697
Product DescriptionRecombinant Human Serine/arginine-rich splicing factor 3 (SRSF3) Protein is expressed from E.coli with N-terminal 6xHis-GB1-tagged. It contains 1-86aa. [Accession | P84103].
Uniprot No.P84103
Gene NamesSRSF3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-86aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-GB1-tagged
Molecular weight17.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons. Also functions as an adapter involved in mRNA nuclear export.

Biological Context

Subcellular Location: Nucleus; Nucleus speckle; Cytoplasm

Product Specifications

Recombinant Human Serine/arginine-rich splicing factor 3 (SRSF3), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-86aa, with N-terminal 6xHis-GB1-tagged tag, molecular weight 17.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing (PubMed:17036044, PubMed:26876937, PubMed:32440474). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons (PubMed:26876937). Also functions as an adapter involved in mRNA nuclear export (PubMed:11336712, PubMed:18364396, PubMed:28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244)

Subcellular Location

Nucleus; Nucleus speckle; Cytoplasm

Subunit

Interacts with CPSF6 (PubMed:15169763). Interacts with RBMY1A1 (PubMed:15595951). Interacts with SREK1/SFRS12 (PubMed:10757789). Interacts with NXF1 (PubMed:12667464, PubMed:17036044). Interacts with YTHDC1, leading to recruitment to RNA elements adjacent to m6A sites (PubMed:26876937, PubMed:28984244). Interacts with SRSP; increases SRSF3 binding to specific exons (PubMed:32440474)

Gene: SRSF3  |  Organism: Homo sapiens  |  Synonyms: Pre-mRNA-splicing factor SRP20; Splicing factor, arginine/serine-rich 3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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