Recombinant Human Serotonin N-acetyltransferase (AANAT), Homo sapiens (Human), E.coli, N-terminal 10xHis-GST-tagged, 51.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human Serotonin N-acetyltransferase (AANAT)

Recombinant Human Serotonin N-acetyltransferase (AANAT) — Controls the night/day rhythm of melatonin production in the pineal gland. Purity >85%.

SKU: BCRECP-000001 Categories: ,
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Product Specifications

Product SkuBCRECP-000001
Product DescriptionRecombinant Human Serotonin N-acetyltransferase (AANAT) Protein is expressed from E.coli with N-terminal 10xHis-GST-tagged. It contains 1-207aa. [Accession | Q16613].
Uniprot No.Q16613
Gene NamesAANAT
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-207aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-GST-tagged
Molecular weight51.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

Biological Context

Subcellular Location: Cytoplasm
Tissue Specificity: Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland
Pathway: Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2

Product Specifications

Recombinant Human Serotonin N-acetyltransferase (AANAT) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-207aa, with N-terminal 10xHis-GST-tagged tag, molecular weight 51.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin

Subcellular Location

Cytoplasm

Tissue Specificity

Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland

Subunit

Monomer (By similarity). Interacts with several 14-3-3 proteins, including YWHAB, YWHAE, YWHAG and YWHAZ, preferentially when phosphorylated at Thr-31. Phosphorylation on Ser-205 also allows binding to YWHAZ, but with lower affinity. The interaction with YWHAZ considerably increases affinity for arylalkylamines and acetyl-CoA and protects the enzyme from dephosphorylation and proteasomal degradation (By similarity). It may also prevent thiol-dependent inactivation (By similarity)

Pathway

Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2

Gene: AANAT  |  Organism: Homo sapiens  |  Synonyms: Aralkylamine N-acetyltransferase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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