Recombinant Human Signal peptidase complex catalytic subunit SEC11C (SEC11C), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 32.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Signal peptidase complex catalytic subunit SEC11C (SEC11C), partial

Recombinant Human Signal peptidase complex catalytic subunit SEC11C (SEC11C), partial — Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmi… Purity >…

SKU: BCRECP-001597 Categories: , ,
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Product Specifications

Product SkuBCRECP-001597
Product DescriptionRecombinant Human Signal peptidase complex catalytic subunit SEC11C (SEC11C) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 49-192aa. [Accession | Q9BY50].
Uniprot No.Q9BY50
Gene NamesSEC11C
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region49-192aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight32.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Human Signal peptidase complex catalytic subunit SEC11C (SEC11C), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 49-192aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 32.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (PubMed:34388369). Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids (PubMed:34388369)

Subcellular Location

Endoplasmic reticulum membrane (Single-pass type II membrane protein)

Subunit

Component of the signal peptidase complex paralog C (SPC-C) composed of a catalytic subunit SEC11C and three accessory subunits SPCS1, SPCS2 and SPCS3 (PubMed:34388369). Within the complex, interacts with SPCS2 and SPCS3 (PubMed:34388369). The complex induces a local thinning of the ER membrane which is used to measure the length of the signal peptide (SP) h-region of protein substrates (PubMed:34388369). This ensures the selectivity of the complex towards h-regions shorter than 18-20 amino acids (PubMed:34388369)

Gene: SEC11C  |  Organism: Homo sapiens  |  Synonyms: Microsomal signal peptidase 21 kDa subunit; SEC11 homolog C; SEC11-like protein 3; SPC21

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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