Recombinant Human SLP adapter and CSK-interacting membrane protein (SCIMP) Protein

Function

Lipid tetraspanin-associated transmembrane adapter/mediator that acts as a scaffold for Src-family kinases and other signaling proteins in immune cells (PubMed:21930792). It is involved in major histocompatibility complex class II (MHC-II) signaling transduction in B cells, where it is required in generating the calcium response and enhancing ERK activity upon MHC-II stimulation (PubMed:21930792). In dendritic cells, it is involved in sustaining CLEC7A/DECTIN1 signaling after CLEC7A activation by fungal beta-glucans (By similarity). It also acts as an agonist-inducible signaling adapter for TLR1, TLR2, TLR3, TLR4, and TLR7 by selectively enabling the expression of pro-inflammatory cytokines IL6 and IL12B in macrophages and acting as a scaffold for phosphorylation of Toll-like receptors by Src-family kinases (By similarity)

Subcellular Location

Cell membrane (Single-pass membrane protein); Cell membrane (Lipid-anchor); Cytoplasmic vesicle, phagosome; Cell projection, ruffle; Cell projection, filopodium

Tissue Specificity

Expressed in antigen-presenting cells, like peripheral blood leukocytes and monocyte-derived dendritic cells (MDDC) (at protein level) (PubMed:21930792). Highly expressed in lymph nodes and spleen. Expressed in antigen-presenting cells (PubMed:21930792). Faintly expressed in the majority of nonimmune system tissues (PubMed:21930792)

Subunit

Interacts with CD37, CD53 and CD81 (PubMed:21930792). Interacts (via proline-rich region) with LYN (via SH3 domain) (PubMed:21930792). Interacts with CSK (via SH2 domain); this interaction is dependent on phosphorylation of Tyr-107 (PubMed:21930792). Interacts with BLNK (via SH2 domain); this interaction is dependent on phosphorylation of Tyr-131 (PubMed:21930792). Interacts with GRB2 (via SH2 domain); this interaction may be dependent on phosphorylation of Tyr-69 (By similarity). Interacts with TLR4; this interaction occurs upon lipopolysaccharide activation of TLR4 and is enhanced by phosphorylation of Tyr-107 by LYN (By similarity). This interaction facilitates the phosphorylation of TLR4 by LYN which elicits a selective cytokine response in macrophages (By similarity)

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• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
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• Mural R.J. et al. PubMed: CI-5GBD0VIIJ7C63 (SEP-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Draber P. et al. SCIMP, a transmembrane adapter protein involved in major histocompatibility complex class II signaling. — Mol. Cell. Biol. (2011)