Recombinant Human Small ribosomal subunit protein uS5 (RPS2)

Function

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (PubMed:23636399). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (PubMed:23636399). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:23636399). Plays a role in the assembly and function of the 40S ribosomal subunit (By similarity). Mutations in this protein affects the control of translational fidelity (By similarity). Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (By similarity)

Subcellular Location

Cytoplasm; Nucleus, nucleolus

Subunit

Component of the small ribosomal subunit (PubMed:23636399). Interacts with zinc finger protein ZNF277 (via zinc-finger domains); the interaction is direct; the interaction is extra-ribosomal (PubMed:30530495). Interaction with ZNF277 competes with the binding of RPS2 to protein arginine methyltransferase PRMT3 (PubMed:30530495). Interacts with PRMT3 (PubMed:27697862). Interacts with PDCD2; the interaction is direct, occurs cotranslationally, and serves to chaperone uS5 to assembly sites in the nucleus (PubMed:33245768). Interacts with PDCD2L; for chaperone function of PDC2L (PubMed:27697862). Interacts (when monoubiquitinated at Lys-54 and Lys-58) with RIOK3 (PubMed:39947182, PubMed:39947183)

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• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Mural R.J. et al. PubMed: CI-5GBD0VIIJ7C63 (SEP-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Vladimirov S.N. et al. Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry. — Eur. J. Biochem. (1996)
• Andersen J.S. et al. Proteomic characterization of the human centrosome by protein correlation profiling. — Nature (2003)
• Swiercz R. et al. Ribosomal protein S2 is a substrate for mammalian PRMT3 (protein arginine methyltransferase 3). — Biochem. J. (2005)
• Dephoure N. et al. A quantitative atlas of mitotic phosphorylation. — Proc. Natl. Acad. Sci. U.S.A. (2008)