Recombinant Human Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 (SVEP1), partial

Function

Required for morphological development, cell alignment and migration of lymphatic endothelial cells during embryonic development, potentially via modulation of ANGPT2-TIE1 signaling and subsequent activation of FOXC2 transcription (By similarity). Required for embryonic lymphatic vascular development, via mediating the correct formation of the first lymphovenous contact site and tight association of the lymphatic endothelium with the venous endothelium (By similarity). Represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells, via its interaction with integrins, thereby inhibiting vasocontraction (PubMed:35802072). Promotes platelet activation, via its interaction with PEAR1 and subsequent activation of AKT/mTOR signaling (PubMed:36792666). Plays a role in epidermal development and keratinocyte differentiation, independent of cell-cell adhesion (PubMed:27892606). May play a role in initial cell attachment of stromal osteogenic cells (By similarity). May promote myoblast cell adhesion when in the presence of integrin ITGA9:ITGB1 (By similarity)

Subcellular Location

Secreted; Nucleus; Cytoplasm; Membrane (Peripheral membrane protein)

Tissue Specificity

Expressed in mesenchymal cells (at protein level) (PubMed:16206243). Expressed in vascular smooth muscle cells (at protein level) (PubMed:35802072). Expressed throughout the epidermis, expression is most prominent in the basal and lower suprabasal layers of the epidermis and in dermal fibroblasts in the upper dermis (at protein level) (PubMed:27892606). Abundantly expressed in the placenta, weakly expressed in heart and skeletal muscle (PubMed:11062057, PubMed:27892606). Also expressed in the lung, adrenal gland and cerebellum (PubMed:27892606)

Subunit

Interacts (via Sushi domain 21) with ITGA9:ITGB1; thereby inhibits Ca(2+) intracellular signaling and as a result represses vasocontraction (PubMed:35802072). Interacts (via Sushi domain 21) with ITGA4:ITGB1; thereby inhibits Ca(2+) intracellular signaling and as a result represses vasocontraction (PubMed:35802072). Interacts with ANGPT1 and ANGPT2 (By similarity). Interacts with PEAR1 (via extracellular domain) (PubMed:36792666). Interacts with HSPG2, TLN1, FN1, COPA, CCT2, IQGAP1, LAMC1 and NID1 (By similarity). Interacts (via C-terminus) with TIE1 (PubMed:37097004)

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• Gilges D. et al. Polydom: a secreted protein with pentraxin, complement control protein, epidermal growth factor and von Willebrand factor A domains. — Biochem. J. (2000)