Recombinant Human Synaptotagmin-1 (SYT1), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 40.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Synaptotagmin-1 (SYT1), partial

Recombinant Human Synaptotagmin-1 (SYT1), partial — Calcium sensor that participates in triggering neurotransmitter release at the synapse. Purity >90%.

SKU: BCRECP-001725 Category:
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Product Specifications

Product SkuBCRECP-001725
Product DescriptionRecombinant Human Synaptotagmin-1 (SYT1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 99-416aa. [Accession | P21579].
Uniprot No.P21579
Gene NamesSYT1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region99-416aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight40.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Calcium sensor that participates in triggering neurotransmitter release at the synapse. May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.

Biological Context

Subcellular Location: Cytoplasmic vesicle, secretory vesicle membrane (Single-pass membrane protein); Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane (Single-pass membrane protein); Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane (Single-pass membrane protein); Cytoplasm
Tissue Specificity: Expressed in melanocytes
Disease Association: Baker-Gordon syndrome (BAGOS) : An autosomal dominant neurodevelopmental disorder characterized by infantile hypotonia, congenital ophthalmic abnormalities, involuntary and hyperkinetic movements, stereotypic behavior, poor or absent speech, EEG abnormalities, and global developmental delay varying in severity from moderate to profound. Behavioral characteristics include sleep disturbance and episodic agitation. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Synaptotagmin-1 (SYT1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 99-416aa, with N-terminal 6xHis-tagged tag, molecular weight 40.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Calcium sensor that participates in triggering neurotransmitter release at the synapse (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003)

Subcellular Location

Cytoplasmic vesicle, secretory vesicle membrane (Single-pass membrane protein); Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane (Single-pass membrane protein); Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane (Single-pass membrane protein); Cytoplasm

Disease Association

Baker-Gordon syndrome (BAGOS) : An autosomal dominant neurodevelopmental disorder characterized by infantile hypotonia, congenital ophthalmic abnormalities, involuntary and hyperkinetic movements, stereotypic behavior, poor or absent speech, EEG abnormalities, and global developmental delay varying in severity from moderate to profound. Behavioral characteristics include sleep disturbance and episodic agitation. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in melanocytes (PubMed:23999003)

Subunit

Homotetramer (Probable). Heterodimer; heterodimerizes with SYT2 in presence of calcium (By similarity). Interacts with SCAMP5 (PubMed:19234194). Interacts with STON2 (PubMed:11381094). Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity). Interacts with SV2A, SV2B and SV2C (By similarity). Interacts with RIMS1 (By similarity). Interacts with PRRT2 (By similarity). Interacts with DNAJC5 in a phosphorylation-dependent manner (By similarity). Interacts (via N-terminus) with RAB3A (By similarity). Interacts with SYT12 (By similarity). Interacts with calmodulin (By similarity). Interacts with DNM1 (via C-terminal proline-rich domain (PRD)); this interaction facilitates vesicle fission during clathrin-mediated endocytosis (CME) (By similarity)

Gene: SYT1  |  Organism: Homo sapiens  |  Synonyms: Synaptotagmin I; p65
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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