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Recombinant Human Talin-1 (TLN1), partial

Recombinant Human Talin-1 (TLN1), partial — High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Purity >90%.

SKU: BCRECP-001778 Category:
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Product Specifications

Product SkuBCRECP-001778
Product DescriptionRecombinant Human Talin-1 (TLN1) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 92-399aa. [Accession | Q9Y490].
Uniprot No.Q9Y490
Gene NamesTLN1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region92-399aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight62.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims.

Biological Context

Subcellular Location: Cell projection, ruffle membrane (Peripheral membrane protein); Cytoplasm, cytoskeleton; Cell surface; Cell junction, focal adhesion
Tissue Specificity: Expressed at low to non-detectable levels in many tissues but highly expressed in skin and pancreas with other tissues including kidney cortex, endocervix, testis, pituitary, liver, and spleen also showing robust expression

Product Specifications

Recombinant Human Talin-1 (TLN1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 92-399aa, with N-terminal GST-tagged tag, molecular weight 62.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims

Subcellular Location

Cell projection, ruffle membrane (Peripheral membrane protein); Cytoplasm, cytoskeleton; Cell surface; Cell junction, focal adhesion

Tissue Specificity

Expressed at low to non-detectable levels in many tissues but highly expressed in skin and pancreas with other tissues including kidney cortex, endocervix, testis, pituitary, liver, and spleen also showing robust expression

Subunit

(Microbial infection) Interacts with human cytomegalovirus protein UL135

Gene: TLN1  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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