Recombinant Human Tissue factor (F3), partial, Homo sapiens (Human), Mammalian cell, C-terminal hFc1-tagged, 53.7kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Tissue factor (F3), partial

Recombinant Human Tissue factor (F3), partial — Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. Purity >95%.

SKU: BCRECP-000618 Category:
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Product Specifications

Product SkuBCRECP-000618
Product DescriptionRecombinant Human Tissue factor (F3) Protein is expressed from Mammalian cell with C-terminal hFc1-tagged. It contains 33-251aa. [Accession | P13726].
Uniprot No.P13726
Gene NamesF3
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region33-251aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-tagged
Molecular weight53.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Lung, placenta and pancreas

Product Specifications

Recombinant Human Tissue factor (F3), partial is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 33-251aa, with C-terminal hFc1-tagged tag, molecular weight 53.7kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade

Subcellular Location

Secreted

Tissue Specificity

Lung, placenta and pancreas

Subunit

Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII

Gene: F3  |  Organism: Homo sapiens  |  Synonyms: Coagulation factor III; Thromboplastin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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