Recombinant Human Translocator protein (TSPO) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 20.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Translocator protein (TSPO) Protein

Recombinant Human Translocator protein (TSPO) Protein — Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme. Purity >85%.

SKU: BCRECP-00071TM Category:
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Product Specifications

Uniprot No.P30536
Gene NamesTSPO
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region2-169aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight20.2kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme. Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism , but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis.

Biological Context

Subcellular Location: Mitochondrion membrane (Multi-pass membrane protein)
Tissue Specificity: Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids

Product Specifications

Recombinant Human Translocator protein (TSPO) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 2-169aa, with N-terminal 10xHis-tagged tag, molecular weight 20.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:24814875), but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides (PubMed:1847678)

Subcellular Location

Mitochondrion membrane (Multi-pass membrane protein)

Tissue Specificity

Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids

Subunit

Interacts with TSPOAP1 (By similarity). May interact with STAR (By similarity). Interacts with MOST-1. Interacts with TMEM97 (PubMed:37047353). Forms a complex with TMEM97 and PGRMC1; the interaction occurs in MIA PaCa-2 cells but not in MCF7 cells (PubMed:37047353)

Gene: TSPO  |  Organism: Homo sapiens  |  Synonyms: Mitochondrial benzodiazepine receptor; PKBS; Peripheral-type benzodiazepine receptor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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