Recombinant Human Transmembrane protein 65 (TMEM65), partial Protein, Homo sapiens(Human), in vitro E.coli expression system, N-terminal 6xHis-SUMO-tagged, 35.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Transmembrane protein 65 (TMEM65), partial Protein

Recombinant Human Transmembrane protein 65 (TMEM65), partial Protein — Mitochondrial protein that plays a critical role in the sodium-dependent calcium efflux from mitochondrion. Purity >90%.

SKU: BCRECP-00608TM Category:
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Product Specifications

Uniprot No.Q6PI78
Gene NamesTMEM65
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region63-240aa
SpeciesHomo sapiens(Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight35.1kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Mitochondrial protein that plays a critical role in the sodium-dependent calcium efflux from mitochondrion. The precise role of TMEM65 in mitochondrial sodium/calcium exchange is however unclear: TMEM65 associates with the SLC8B1/NCLX antiporter and probably promotes the sodium/calcium exchange mediated by SLC8B1/NCLX. According to another report, TMEM65 functions as the mitochondrial sodium/calcium antiporter itself: homodimerization drives formation of a pocket capable of binding calcium.

Biological Context

Subcellular Location: Mitochondrion inner membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Predominantly expressed the ventricular tissue (at protein level)
Disease Association: [Defects in TMEM65 may cause a mitochondrial disorder characterized by a complex encephalomyopathic phenotype. Clinical features includ microcephaly, dysmorphic features, psychomotor regression, hypotonia, growth retardation, lactic acidosis, intractable seizures, dyskenetics movements, without cardiomyopathy ]

Product Specifications

Recombinant Human Transmembrane protein 65 (TMEM65), partial Protein is a recombinant protein from Homo sapiens(Human), expressed in in vitro E.coli expression system, covering amino acids 63-240aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 35.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Mitochondrial protein that plays a critical role in the sodium-dependent calcium efflux from mitochondrion (PubMed:40200126, PubMed:40691517). The precise role of TMEM65 in mitochondrial sodium/calcium exchange is however unclear: TMEM65 associates with the SLC8B1/NCLX antiporter and probably promotes the sodium/calcium exchange mediated by SLC8B1/NCLX (PubMed:40200126). According to another report, TMEM65 functions as the mitochondrial sodium/calcium antiporter itself: homodimerization drives formation of a pocket capable of binding calcium (PubMed:40691517). Essential for maintaining proper cardiac intercalated disk (ICD) structure and function as well as cardiac conduction velocity in the heart (By similarity). Its association with SCN1B is required for stabilizing the perinexus in the ICD and for localization of GJA1 and SCN5A to the ICD (By similarity)

Subcellular Location

Mitochondrion inner membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein)

Disease Association

[Defects in TMEM65 may cause a mitochondrial disorder characterized by a complex encephalomyopathic phenotype. Clinical features includ microcephaly, dysmorphic features, psychomotor regression, hypotonia, growth retardation, lactic acidosis, intractable seizures, dyskenetics movements, without cardiomyopathy (PubMed:28295037)]

Tissue Specificity

Predominantly expressed the ventricular tissue (at protein level)

Subunit

Monomer (By similarity). Homodimer (PubMed:26403541, PubMed:40691517). Interacts with SLC8B1/NCLX; required for SLC8B1/NCLX antiporter activity (PubMed:40200126). Interacts with GJA1 (By similarity). Interacts weakly with DSP (By similarity). Interacts with SCN1B (By similarity)

Gene: TMEM65  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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