Recombinant Human Trimethylguanosine synthase (TGS1), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 31.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Trimethylguanosine synthase (TGS1), partial

Recombinant Human Trimethylguanosine synthase (TGS1), partial — Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. Purity >90%.

SKU: BCRECP-001771 Categories: ,
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Product Specifications

Product SkuBCRECP-001771
Product DescriptionRecombinant Human Trimethylguanosine synthase (TGS1) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 713-853aa. [Accession | Q96RS0].
Uniprot No.Q96RS0
Gene NamesTGS1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region713-853aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight31.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs).

Biological Context

Subcellular Location: Cytoplasm; Nucleus, Cajal body; Nucleus, nucleolus
Tissue Specificity: Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta

Product Specifications

Recombinant Human Trimethylguanosine synthase (TGS1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 713-853aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 31.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation

Subcellular Location

Cytoplasm; Nucleus, Cajal body; Nucleus, nucleolus

Tissue Specificity

Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta

Subunit

May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN

Gene: TGS1  |  Organism: Homo sapiens  |  Synonyms: CLL-associated antigen KW-2; Cap-specific guanine-N(2) methyltransferase; Hepatocellular carcinoma-associated antigen 137; Nuclear receptor coactivator 6-interacting protein; PRIP-interacting protein with methyltransferase motif
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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