Recombinant Human Trophoblast glycoprotein (TPBG), partial (Active), Homo sapiens (Human), Mammalian cell, N-terminal 10xHis-tagged, 38.9 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Trophoblast glycoprotein (TPBG), partial (Active)

Recombinant Human Trophoblast glycoprotein (TPBG), partial (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-000213 Category:
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Product SKUBCACP-000213
Product DescriptionRecombinant Human Trophoblast glycoprotein (TPBG) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged. It contains 32-355aa. [Accession | Q13641].
Uniprot No.Q13641
Gene NamesTPBG
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region32-355aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight38.9 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human DKK1 at 2 μg/mL can bind Anti-DKK1 recombinant antibody , the EC50 is 1.283-2.544 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(5T4 oncofetal antigen)(5T4 oncofetal trophoblast glycoprotein)(5T4 oncotrophoblast glycoprotein)(M6P1)(Wnt-activated inhibitory factor 1)(WAIF1)

Function

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma

Product Specifications

Recombinant Human Trophoblast glycoprotein (TPBG), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 32-355aa, with N-terminal 10xHis-tagged tag, molecular weight 38.9 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Tissue Specificity

Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma

Gene: TPBG  |  Organism: Homo sapiens  |  Synonyms: 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; M6P1; Wnt-activated inhibitory factor 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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