Product Specifications
| Catalog Number | BIC-5362E |
|---|---|
| Target | Tumor necrosis factor |
| Target Symbol | TNF |
| Species | Human |
| Expression System | CHO |
| Expression Region | Val77-Leu233 |
| Molecular Weight | 17.4 kDa |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Formulation | Lyophilized |
| Buffer | PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution; Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping | The product is shipped with blue ice. |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Accession | P01375 |
| Alternative Names | Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2 |
Bioactivity
Recombinant Human TNFα (Cat. No. BIC-5362E) measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL. TheBioactivity of BIC-5362E was equivalent to other competing products.
Quality Data
Recombinant Human TNFα (Cat. No. BIC-5362E) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with BIC-5362E SDS-PAGE.
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
