Recombinant Human Tumor necrosis factor (TNF), partial (Active), Homo sapiens (Human), Yeast, N-terminal 6xHis-tagged, 19.4 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Tumor necrosis factor (TNF), partial (Active)

Recombinant Human Tumor necrosis factor (TNF), partial (Active) — recombinant protein from Homo sapiens (Human), expressed in Yeast. Purity >90%.

SKU: BCACP-000041 Categories: ,
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Product SKUBCACP-000041
Product DescriptionRecombinant Human Tumor necrosis factor (TNF) protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 77-233aa. [Accession | P01375].
Uniprot No.P01375
Gene NamesTNF
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region77-233aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight19.4 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human TNF at 5 μg/mL can bind Human TNFR2 protein . The EC50 is 3.470-4.107 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesAPC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; Tnf; TNF superfamily member 2; TNF superfamily, member 2; TNF, macrophage derived; TNF, monocyte derived; TNF-a; TNF-alpha; TNFA; TNFA_HUMAN; TNFSF2; Tumor necrosis factor (TNF superfamily member 2); Tumor necrosis factor alpha; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor, Membrane Form; Tumor necrosis factor, soluble form

Function

The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Biological Context

Subcellular Location: Secreted
Disease Association: Psoriatic arthritis (PSORAS) : An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). [Disease susceptibility is associated with variants affecting the gene represented in this entry] | Immunodeficiency 127 (IMD127) : An autosomal recessive immunologic disorder characterized by increased susceptibility to pulmonary infection with Mycobacterium tuberculosis. Affected individuals develop recurrent pulmonary tuberculosis, but have no adverse reaction to live BCG vaccination. [The disease may be caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Tumor necrosis factor (TNF), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Yeast, covering amino acids 77-233aa, with N-terminal 6xHis-tagged tag, molecular weight 19.4 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TNF at 5 μg/mL can bind Human TNFR2 protein . The EC50 is 3.470-4.107 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells

Subcellular Location

Secreted

Disease Association

Psoriatic arthritis (PSORAS) : An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). [Disease susceptibility is associated with variants affecting the gene represented in this entry] | Immunodeficiency 127 (IMD127) : An autosomal recessive immunologic disorder characterized by increased susceptibility to pulmonary infection with Mycobacterium tuberculosis. Affected individuals develop recurrent pulmonary tuberculosis, but have no adverse reaction to live BCG vaccination. [The disease may be caused by variants affecting the gene represented in this entry]

Subunit

Homotrimer. Interacts with SPPL2B

Gene: TNF  |  Organism: Homo sapiens  |  Synonyms: Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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