Recombinant Human Ubiquitin-conjugating enzyme E2 J1 (UBE2J1), partial, Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 32.5kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Ubiquitin-conjugating enzyme E2 J1 (UBE2J1), partial

Recombinant Human Ubiquitin-conjugating enzyme E2 J1 (UBE2J1), partial — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >95%.

SKU: BCRECP-001885 Categories: ,
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Product Specifications

Product SkuBCRECP-001885
Product DescriptionRecombinant Human Ubiquitin-conjugating enzyme E2 J1 (UBE2J1) Protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 1-282aa. [Accession | Q9Y385].
Uniprot No.Q9Y385
Gene NamesUBE2J1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region1-282aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight32.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

(Microbial infection) Promotes Dengue virus RNA replication by negatively regulating IFN-beta signaling and mediating 'Lys-48'-linked ubiquitination on IRF3.

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Single-pass type IV membrane protein)
Tissue Specificity: Expressed in testes
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Human Ubiquitin-conjugating enzyme E2 J1 (UBE2J1), partial is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 1-282aa, with C-terminal 10xHis-tagged tag, molecular weight 32.5kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

(Microbial infection) Promotes Dengue virus RNA replication by negatively regulating IFN-beta signaling and mediating 'Lys-48'-linked ubiquitination on IRF3 (PubMed:30157886)

Subcellular Location

Endoplasmic reticulum membrane (Single-pass type IV membrane protein)

Tissue Specificity

Expressed in testes

Subunit

Component of the HRD1 complex, which comprises at least SYNV1/HRD1, DERL1/2, FAM8A1, HERPUD1/HERP, OS9, SEL1L and UBE2J1 (PubMed:28827405). Interacts with E3 ligase RNF26 (PubMed:33472082). Interacts with E3 ligase RNF133 (PubMed:35831855)

Pathway

Protein modification; protein ubiquitination

Gene: UBE2J1  |  Organism: Homo sapiens  |  Synonyms: E2 ubiquitin-conjugating enzyme J1; Non-canonical ubiquitin-conjugating enzyme 1; Yeast ubiquitin-conjugating enzyme UBC6 homolog E
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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