Recombinant Human Unconventional myosin-X (MYO10), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 36.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Unconventional myosin-X (MYO10), partial

Recombinant Human Unconventional myosin-X (MYO10), partial — Functions as a dominant-negative regulator of isoform 1, suppressing its filopodia-inducing and axon outgrowth-promoting activities. Purity >90%.

SKU: BCRECP-001214 Category:
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Product Specifications

Product SkuBCRECP-001214
Product DescriptionRecombinant Human Unconventional myosin-X (MYO10) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1669-1917aa. [Accession | Q9HD67].
Uniprot No.Q9HD67
Gene NamesMYO10
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1669-1917aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight36.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Functions as a dominant-negative regulator of isoform 1, suppressing its filopodia-inducing and axon outgrowth-promoting activities. In hippocampal neurons, it increases VASP retention in spine heads to induce spine formation and spine head expansion.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Cell projection, lamellipodium; Cell projection, ruffle; Cytoplasm, cytoskeleton; Cell projection, filopodium tip; Cytoplasm, cell cortex; Cell projection, filopodium membrane (Peripheral membrane protein)
Tissue Specificity: Ubiquitous

Product Specifications

Recombinant Human Unconventional myosin-X (MYO10), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1669-1917aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 36.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Functions as a dominant-negative regulator of isoform 1, suppressing its filopodia-inducing and axon outgrowth-promoting activities. In hippocampal neurons, it increases VASP retention in spine heads to induce spine formation and spine head expansion (By similarity)

Subcellular Location

Cytoplasm, cytosol; Cell projection, lamellipodium; Cell projection, ruffle; Cytoplasm, cytoskeleton; Cell projection, filopodium tip; Cytoplasm, cell cortex; Cell projection, filopodium membrane (Peripheral membrane protein)

Tissue Specificity

Ubiquitous

Subunit

Monomer, when in an inactive conformation in the cytosol. Homodimer in its active, membrane-bound conformation; antiparallel coiled coil-mediated dimer formation. Interacts strongly with CALM3 and weakly with CALM, the CALM3 interaction is essential for function in filopodial extension and motility. Interacts with ECPAS. Interacts with NEO1. Interacts with ITGB1 and ITGB3. Interacts with VASP (By similarity). Interacts with DCC and ITGB5; the presence of DCC inhibits ITGB5 binding. Interacts with tubulin; ITGB5 or DCC binding inhibits tubulin binding

Gene: MYO10  |  Organism: Homo sapiens  |  Synonyms: Unconventional myosin-10
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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