Recombinant Human Valacyclovir hydrolase (BPHL), Homo sapiens (Human), Yeast, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 32.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Valacyclovir hydrolase (BPHL)

Recombinant Human Valacyclovir hydrolase (BPHL) — Specific alpha-amino acid ester serine hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a pr… Purity >90%.

SKU: BCRECP-000172 Categories: ,
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Product Specifications

Product SkuBCRECP-000172
Product DescriptionRecombinant Human Valacyclovir hydrolase (BPHL) Protein is expressed from Yeast with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 38-291aa. [Accession | Q86WA6].
Uniprot No.Q86WA6
Gene NamesBPHL
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region38-291aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight32.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Specific alpha-amino acid ester serine hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. Has homocysteine-thiolactonase activity (in vitro) and may play a significant role in the detoxification of homocysteine thiolactone in vivo. Catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir, converting them into their active forms (acyclovir and ganciclovir).

Biological Context

Subcellular Location: Mitochondrion
Tissue Specificity: Expressed at high levels in liver and kidney and lower levels in heart, intestine and skeletal muscle

Product Specifications

Recombinant Human Valacyclovir hydrolase (BPHL) is a recombinant protein from Homo sapiens (Human), expressed in Yeast, covering amino acids 38-291aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 32.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Specific alpha-amino acid ester serine hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol (PubMed:12732646, PubMed:15832508, PubMed:18256025, PubMed:25333274, PubMed:32196348). Has homocysteine-thiolactonase activity (in vitro) and may play a significant role in the detoxification of homocysteine thiolactone in vivo (PubMed:25333274). Catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir, converting them into their active forms (acyclovir and ganciclovir) (PubMed:15832508, PubMed:18256025)

Subcellular Location

Mitochondrion

Tissue Specificity

Expressed at high levels in liver and kidney and lower levels in heart, intestine and skeletal muscle

Subunit

Monomer. May also form homodimers

Gene: BPHL  |  Organism: Homo sapiens  |  Synonyms: Biphenyl hydrolase-like protein; Biphenyl hydrolase-related protein; Breast epithelial mucin-associated antigen; L-homocysteine-thiolactonase BPHL; Valacyclovir hydrolase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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