Product Specifications
| Catalog Number | BIC-3736E |
|---|---|
| Target | Wnt family member 3A |
| Target Symbol | WNT3A |
| Species | Human |
| Expression System | CHO |
| Expression Region | Ser19-Lys352 with a C-terminal linker GGGGGS and Human lgG1 (Asp104-Lys330) |
| Molecular Weight | 58.2 kDa |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤100 EU/mg by the LAL method. |
| Formulation | Lyophilized |
| Buffer | Lyophilized from a 0.22 μm filtered solution containing PBS, pH7.4. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution; Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping | The product is shipped with blue ice. |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Accession | NP_149122 |
| Alternative Names | MG/VAIL C119418; MG/VAIL C119419; MG/VAIL C119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a |
Bioactivity
Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 5 ng/mL BMP2. The ED50 for this effect is ＜30 ng/mL. The bioactivity of Recombinant Human Wnt-3a (Fc Tag) (Cat. No. BIC-3736E) was higher than other competing products.
Quality Data
Recombinant Human Wnt-3a (Fc Tag) (Cat. No. BIC-3736E) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with BIC-3736E SDS-PAGE.
Highly consistent bioactivity between different production batches of Recombinant Human Wnt-3a (Fc Tag) (Cat. No. BIC-3736E).
