Recombinant Influenza C virus Hemagglutinin-esterase-fusion glycoprotein (HE), partial, Influenza C virus (strain C/Great lakes/1167/1954), E.coli, N-terminal 10xHis-tagged, 54.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Influenza C virus Hemagglutinin-esterase-fusion glycoprotein (HE), partial

Recombinant Influenza C virus Hemagglutinin-esterase-fusion glycoprotein (HE), partial — Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Purity >90%.

SKU: BCRECP-002980 Categories: , , ,
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Product Specifications

Product SkuBCRECP-002980
Product DescriptionRecombinant Influenza C virus Hemagglutinin-esterase-fusion glycoprotein (HE) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 2-433aa. [Accession | P07971].
Uniprot No.P07971
Gene NamesHE
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-433aa
SpeciesInfluenza C virus (strain C/Great lakes/1167/1954)
Tag InfoN-terminal 10xHis-tagged
Molecular weight54.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Influenza C virus Hemagglutinin-esterase-fusion glycoprotein (HE), partial is a recombinant protein from Influenza C virus (strain C/Great lakes/1167/1954), expressed in E.coli, covering amino acids 2-433aa, with N-terminal 10xHis-tagged tag, molecular weight 54.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell (By similarity)

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Subunit

Homotrimer of disulfide-linked HEF1-HEF2

Gene: HE  |  Organism: Influenza C virus (strain C/Great lakes/1167/1954)

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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