Recombinant Klebsiella pneumoniae Beta-lactamase SHV-1 (bla)

Recombinant Klebsiella pneumoniae Beta-lactamase SHV-1 (bla) — Broad-spectrum beta-lactamase which confers resistance to penicillins, as well as first- and third-generation cephalosporins. Purity >90%.

SKU: BCRECP-002210 Category:

Product Specifications

Product SkuBCRECP-002210
Product DescriptionRecombinant Klebsiella pneumoniae Beta-lactamase SHV-1 (bla) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 22-286aa. [Accession | P0AD64].
Uniprot No.P0AD64
Gene Namesbla
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region22-286aa
SpeciesKlebsiella pneumoniae
Tag InfoN-terminal 6xHis-tagged
Molecular weight30.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Broad-spectrum beta-lactamase which confers resistance to penicillins, as well as first- and third-generation cephalosporins. Has nitrocefin-hydrolyzing activity. Inactive against the cephalosporins, cefotaxime and cefoxitin, and the carbapenem, meropenem.

Product Specifications

Recombinant Klebsiella pneumoniae Beta-lactamase SHV-1 (bla) is a recombinant protein from Klebsiella pneumoniae, expressed in Yeast, covering amino acids 22-286aa, with N-terminal 6xHis-tagged tag, molecular weight 30.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Broad-spectrum beta-lactamase which confers resistance to penicillins, as well as first- and third-generation cephalosporins (PubMed:10639363, PubMed:18955486, PubMed:19351161, PubMed:22908166, PubMed:23145056, PubMed:23252553). Has nitrocefin-hydrolyzing activity (PubMed:10231522, PubMed:19351161, PubMed:23252553). Inactive against the cephalosporins, cefotaxime and cefoxitin, and the carbapenem, meropenem (PubMed:10639363, PubMed:19351161)

Subunit

Interacts with S.clavuligerus beta-lactamase inhibitor protein BLIP

Gene: bla  |  Organism: Klebsiella pneumoniae  |  Synonyms: PIT-2; Sulfhydryl reagent variable 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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