Recombinant Lake Victoria marburgvirus Nucleoprotein (NP), partial, Lake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967)), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 50.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Lake Victoria marburgvirus Nucleoprotein (NP), partial

Recombinant Lake Victoria marburgvirus Nucleoprotein (NP), partial — Encapsidates the genome, protecting it from nucleases. Purity >85%.

SKU: BCRECP-003892 Categories: ,
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Product Specifications

Product SkuBCRECP-003892
Product DescriptionRecombinant Lake Victoria marburgvirus Nucleoprotein (NP) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 317-695aa. [Accession | P35263].
Uniprot No.P35263
Gene NamesNP
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region317-695aa
SpeciesLake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967))
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight50.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.

Biological Context

Subcellular Location: Virion; Host cytoplasm

Product Specifications

Recombinant Lake Victoria marburgvirus Nucleoprotein (NP), partial is a recombinant protein from Lake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967)), expressed in E.coli, covering amino acids 317-695aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 50.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity)

Subcellular Location

Virion; Host cytoplasm

Subunit

Homooligomer. Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with VP35 and VP30 to form the nucleocapsid. Also interacts with VP24 and VP40 (By similarity)

Gene: NP  |  Organism: Lake Victoria marburgvirus (strain Popp-67)  |  Synonyms: Nucleocapsid protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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