Recombinant Lake Victoria marburgvirus Polymerase cofactor VP35 (VP35), Lake Victoria marburgvirus (strain Ozolin-75) (MARV) (Marburg virus (strain South Africa/Ozolin/1975)), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 43.7kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Lake Victoria marburgvirus Polymerase cofactor VP35 (VP35)

Recombinant Lake Victoria marburgvirus Polymerase cofactor VP35 (VP35) — Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Purity >95%.

SKU: BCRECP-004736 Categories: ,
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Product Specifications

Product SkuBCRECP-004736
Product DescriptionRecombinant Lake Victoria marburgvirus Polymerase cofactor VP35 (VP35) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-329aa. [Accession | Q6UY68].
Uniprot No.Q6UY68
Gene NamesVP35
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-329aa
SpeciesLake Victoria marburgvirus (strain Ozolin-75) (MARV) (Marburg virus (strain South Africa/Ozolin/1975))
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight43.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane.

Biological Context

Subcellular Location: Virion; Host cytoplasm

Product Specifications

Recombinant Lake Victoria marburgvirus Polymerase cofactor VP35 (VP35) is a recombinant protein from Lake Victoria marburgvirus (strain Ozolin-75) (MARV) (Marburg virus (strain South Africa/Ozolin/1975)), expressed in E.coli, covering amino acids 1-329aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 43.7kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane (By similarity)

Subcellular Location

Virion; Host cytoplasm

Subunit

Homotetramer. Interacts with L polymerase; this complex constitutes a functional polymerase complex. Homomultimerization via the coiled coil domain is a prerequisite for binding to L. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein (By similarity). Interacts with NP (PubMed:28566377, PubMed:28659479). Disrupts innate immune signaling in infected host cell (By similarity)

Gene: VP35  |  Organism: Lake Victoria marburgvirus (strain Ozolin-75)  |  Synonyms: Marburg VP35

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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