Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S), partial, Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 48.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S), partial

Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S), partial — Mediates the entry of L. Purity >85%.

SKU: BCRECP-003178 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-003178
Product DescriptionRecombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 32-414aa(S192N,Y369S). [Accession | P0DJM0].
Uniprot No.P0DJM0
Gene NamesinlA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region32-414aa(S192N,Y369S)
SpeciesListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight48.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Mediates the entry of L.monocytogenes into host intestinal epithelial cells; transformation with inlA alone allows L.innocua (a non-invasive species) to be taken up by host cells. Binds to human receptor cadherin-1 (E-cadherin, CDH1); the chicken homolog of cadherin-1 but not cadherin-2 function as receptors. Mouse cadherin-1 is not a receptor, however mutating a single surface-exposed residue (Glu-172 to Pro in mouse) allows cadherin-1 to act as a receptor for InlA.

Biological Context

Subcellular Location: Secreted, cell wall (Peptidoglycan-anchor); Secreted

Product Specifications

Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S), partial is a recombinant protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e), expressed in E.coli, covering amino acids 32-414aa(S192N,Y369S), with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 48.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Mediates the entry of L.monocytogenes into host intestinal epithelial cells; transformation with inlA alone allows L.innocua (a non-invasive species) to be taken up by host cells (PubMed:10406800, PubMed:1905979, PubMed:8601315). Binds to human receptor cadherin-1 (E-cadherin, CDH1); the chicken homolog of cadherin-1 but not cadherin-2 function as receptors (PubMed:8601315). Mouse cadherin-1 is not a receptor, however mutating a single surface-exposed residue (Glu-172 to Pro in mouse) allows cadherin-1 to act as a receptor for InlA (PubMed:10406800)

Subcellular Location

Secreted, cell wall (Peptidoglycan-anchor); Secreted

Subunit

Interacts with host (human) cadherin-1 (CDH1) (PubMed:12526809, PubMed:17540170, PubMed:17715295). The formation of the complex between inlA and cadherin-1 is calcium-dependent (PubMed:12526809). Mutagenesis studies show it is possible to increase the affinity of InlA for CDH1 by rational engineering of InlA residues (PubMed:17715295)

Gene: inlA  |  Organism: Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top