Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S), partial, Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 48.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S), partial

Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S), partial — Mediates the entry of L. Purity >85%.

SKU: BCRECP-003178 Category:
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Product Specifications

Product SkuBCRECP-003178
Product DescriptionRecombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 32-414aa(S192N,Y369S). [Accession | P0DJM0].
Uniprot No.P0DJM0
Gene NamesinlA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region32-414aa(S192N,Y369S)
SpeciesListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight48.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Mediates the entry of L.monocytogenes into host intestinal epithelial cells; transformation with inlA alone allows L.innocua (a non-invasive species) to be taken up by host cells. Binds to human receptor cadherin-1 (E-cadherin, CDH1); the chicken homolog of cadherin-1 but not cadherin-2 function as receptors. Mouse cadherin-1 is not a receptor, however mutating a single surface-exposed residue (Glu-172 to Pro in mouse) allows cadherin-1 to act as a receptor for InlA.

Biological Context

Subcellular Location: Secreted, cell wall (Peptidoglycan-anchor); Secreted

Product Specifications

Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA) (S192N,Y369S), partial is a recombinant protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e), expressed in E.coli, covering amino acids 32-414aa(S192N,Y369S), with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 48.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Mediates the entry of L.monocytogenes into host intestinal epithelial cells; transformation with inlA alone allows L.innocua (a non-invasive species) to be taken up by host cells (PubMed:10406800, PubMed:1905979, PubMed:8601315). Binds to human receptor cadherin-1 (E-cadherin, CDH1); the chicken homolog of cadherin-1 but not cadherin-2 function as receptors (PubMed:8601315). Mouse cadherin-1 is not a receptor, however mutating a single surface-exposed residue (Glu-172 to Pro in mouse) allows cadherin-1 to act as a receptor for InlA (PubMed:10406800)

Subcellular Location

Secreted, cell wall (Peptidoglycan-anchor); Secreted

Subunit

Interacts with host (human) cadherin-1 (CDH1) (PubMed:12526809, PubMed:17540170, PubMed:17715295). The formation of the complex between inlA and cadherin-1 is calcium-dependent (PubMed:12526809). Mutagenesis studies show it is possible to increase the affinity of InlA for CDH1 by rational engineering of InlA residues (PubMed:17715295)

Gene: inlA  |  Organism: Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Key Publications

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