Recombinant Macaca fascicularis Dipeptidase 3(DPEP3) (Active), Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey), Mammalian cell, C-terminal 10xHis-tagged, 48.5 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Macaca fascicularis Dipeptidase 3(DPEP3) (Active)

Recombinant Macaca fascicularis Dipeptidase 3(DPEP3) (Active) — Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem. Purity >95%.

SKU: BCACP-000275 Categories: , ,
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Product SKUBCACP-000275
Product DescriptionRecombinant Cynomolgus monkey Dipeptidase 3(DPEP3) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 36-463aa. [Accession | Q4R7M2].
Uniprot No.Q4R7M2
Gene NamesDPEP3
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region36-463aa
SpeciesMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Tag InfoC-terminal 10xHis-tagged
Molecular weight48.5 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Anti-PCSK9 recombinant antibody at 2 μg/mL can bind Human PCSK9 protein. The EC50 is 7.450-8.692 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human PCSK9 at 1 μg/mL on streptavidin coated plates can bind Anti-PCSK9 recombinant antibody . The EC50 is 0.3792-1.157 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem. The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide. A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance.

Biological Context

Subcellular Location: Membrane (Lipid-anchor, GPI-anchor)

Product Specifications

Recombinant Macaca fascicularis Dipeptidase 3(DPEP3) (Active) is a recombinant protein from Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey), expressed in Mammalian cell, covering amino acids 36-463aa, with C-terminal 10xHis-tagged tag, molecular weight 48.5 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-PCSK9 recombinant antibody at 2 μg/mL can bind Human PCSK9 protein. The EC50 is 7.450-8.692 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human PCSK9 at 1 μg/mL on streptavidin coated plates can bind Anti-PCSK9 recombinant antibody . The EC50 is 0.3792-1.157 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem (By similarity). The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide (By similarity). A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance (By similarity)

Subcellular Location

Membrane (Lipid-anchor, GPI-anchor)

Subunit

Homodimer; disulfide-linked (By similarity). Interacts with TEX101; co-localized on the cell surface of spermatocytes, spermatids, and testicular spermatozoa, co-localized only in cytoplasmic droplets of caput and corpus epididymal sperm (By similarity)

Gene: DPEP3  |  Organism: Macaca fascicularis

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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