Recombinant Macaca mulatta Neurofilament light chain (NEFL), Macaca mulatta (Rhesus macaque), E.coli, N-terminal 10xHis-tagged, 67.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Macaca mulatta Neurofilament light chain (NEFL)

Recombinant Macaca mulatta Neurofilament light chain (NEFL) — Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Purity >85%.

SKU: BCRECP-003862 Category:
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Product Specifications

Product SkuBCRECP-003862
Product DescriptionRecombinant Macaca mulatta Neurofilament light chain (NEFL) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 2-543aa. [Accession | F7ED68].
Uniprot No.F7ED68
Gene NamesNEFL
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-543aa
SpeciesMacaca mulatta (Rhesus macaque)
Tag InfoN-terminal 10xHis-tagged
Molecular weight67.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front.

Product Specifications

Recombinant Macaca mulatta Neurofilament light chain (NEFL) is a recombinant protein, expressed in E.coli, covering amino acids 2-543aa, with N-terminal 10xHis-tagged tag, molecular weight 67.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips

Gene: NEFL  |  Organism: Macaca mulatta

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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