Recombinant Macrovipera lebetina Chymotrypsin-like protease VLCTLP, Macrovipera lebetina (Levantine viper) (Vipera lebetina), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 33.0kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Macrovipera lebetina Chymotrypsin-like protease VLCTLP

Recombinant Macrovipera lebetina Chymotrypsin-like protease VLCTLP — Snake venom serine protease with tyrosine-specific chymotrypsin-like activity. Purity >95%.

SKU: BCRECP-003723 Categories: , ,
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Product Specifications

Product SkuBCRECP-003723
Product DescriptionRecombinant Macrovipera lebetina Chymotrypsin-like protease VLCTLP Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 25-257aa. [Accession | E0Y421].
Uniprot No.E0Y421
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region25-257aa
SpeciesMacrovipera lebetina (Levantine viper) (Vipera lebetina)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight33.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Snake venom serine protease with tyrosine-specific chymotrypsin-like activity. Hydrolyzes the N-acetyl-L-tyrosine ethyl ester (ATEE). Has weak fibrinogenolytic activity.

Product Specifications

Recombinant Macrovipera lebetina Chymotrypsin-like protease VLCTLP is a recombinant protein, expressed in E.coli, covering amino acids 25-257aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 33.0kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Snake venom serine protease with tyrosine-specific chymotrypsin-like activity. Hydrolyzes the N-acetyl-L-tyrosine ethyl ester (ATEE). Has weak fibrinogenolytic activity. Weakly hydrolyzes azocasein, Aalpha-chain (FGA) and more slowly Bbeta-chain (FGB) of fibrinogen. Optimal substrates are angiotensins I and II (AGT)

Organism: Macrovipera lebetinus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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