Recombinant Measles virus Hemagglutinin glycoprotein (H) Protein, Measles virus (strain Edmonston-AIK-C vaccine) (MeV) (Subacute sclerose panencephalitis virus), in vitro E.coli expression system, N-terminal 10xHis-tagged, 75.4kDa, purity >85% as determined by SDS-PAGE.
,

Recombinant Measles virus Hemagglutinin glycoprotein (H) Protein

Recombinant Measles virus Hemagglutinin glycoprotein (H) Protein — Attaches the virus to the human SLAMF1/CD150 receptor for entry into host dendritic cells, macrophages, activated memory T cells and naive or memory B cells, thereby explaining the long immunosuppr… Purity >85%.

SKU: BCRECP-00065TM Categories: ,
Online Inquiry →

Product Specifications

Uniprot No.P35971
Gene NamesH
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-617aa
SpeciesMeasles virus (strain Edmonston-AIK-C vaccine) (MeV) (Subacute sclerose panencephalitis virus)
Tag InfoN-terminal 10xHis-tagged
Molecular weight75.4kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Attaches the virus to the human SLAMF1/CD150 receptor for entry into host dendritic cells, macrophages, activated memory T cells and naive or memory B cells, thereby explaining the long immunosuppression that follows infection. In the respiratory airways, binds to the NECTIN4 receptor for entry into the host cell. Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion.

Biological Context

Subcellular Location: Virion membrane (Single-pass type II membrane protein); Host cell membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Measles virus Hemagglutinin glycoprotein (H) Protein is a recombinant protein from Measles virus (strain Edmonston-AIK-C vaccine) (MeV) (Subacute sclerose panencephalitis virus), expressed in in vitro E.coli expression system, covering amino acids 1-617aa, with N-terminal 10xHis-tagged tag, molecular weight 75.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Attaches the virus to the human SLAMF1/CD150 receptor for entry into host dendritic cells, macrophages, activated memory T cells and naive or memory B cells, thereby explaining the long immunosuppression that follows infection (By similarity). In the respiratory airways, binds to the NECTIN4 receptor for entry into the host cell (By similarity). Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity). The vaccine and laboratory-adapted strains use host CD46 as an alternate receptor (PubMed:12029158). The high degree of interaction between H and CD46 results in down-regulation of the latter from the surface of infected cells, rendering them more sensitive to c3b-mediated complement lysis (By similarity)

Subcellular Location

Virion membrane (Single-pass type II membrane protein); Host cell membrane (Single-pass type II membrane protein)

Subunit

Homodimer; disulfide-linked (By similarity). Further forms homotetramer (dimer of dimers) (By similarity). Interacts (via C-terminus) with human NECTIN4 (via N-terminus); this interaction allows attachment to the respiratory epithelium and viral entry (By similarity). Interacts (via C-terminus) with human SLAMF1/CD150 (via N-terminus); this interaction allows attachment and viral entry into the CD150-expressing immune cells (Probable). Interacts with human CD46 antigen (PubMed:12029158)

Gene: H  |  Organism: Measles virus (strain Edmonston-AIK-C vaccine)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top