Recombinant Measles virus Phosphoprotein (P/V), partial, Measles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus), E.coli, N-terminal 6xHis-KSI-tagged, 21.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Measles virus Phosphoprotein (P/V), partial

Recombinant Measles virus Phosphoprotein (P/V), partial — Essential cofactor of the RNA polymerase L that plays a central role in the transcription and replication by forming the polymerase complex with RNA polymerase L and recruiting L to the genomic N-R… Purity >90%.

SKU: BCRECP-003990 Categories: ,
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Product Specifications

Product SkuBCRECP-003990
Product DescriptionRecombinant Measles virus Phosphoprotein (P/V) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 459-507aa. [Accession | P03422].
Uniprot No.P03422
Gene NamesP/V
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region459-507aa
SpeciesMeasles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight21.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Essential cofactor of the RNA polymerase L that plays a central role in the transcription and replication by forming the polymerase complex with RNA polymerase L and recruiting L to the genomic N-RNA template for RNA synthesis. Also plays a central role in the encapsidation of nascent RNA chains by forming the encapsidation complex with the nucleocapsid protein N (N-P complex). Acts as a chaperone for newly synthesized free N protein, so-called N0, allowing encapsidation of nascent RNA chains during replication.

Product Specifications

Recombinant Measles virus Phosphoprotein (P/V), partial is a recombinant protein from Measles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus), expressed in E.coli, covering amino acids 459-507aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 21.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Essential cofactor of the RNA polymerase L that plays a central role in the transcription and replication by forming the polymerase complex with RNA polymerase L and recruiting L to the genomic N-RNA template for RNA synthesis (By similarity). Also plays a central role in the encapsidation of nascent RNA chains by forming the encapsidation complex with the nucleocapsid protein N (N-P complex). Acts as a chaperone for newly synthesized free N protein, so-called N0, allowing encapsidation of nascent RNA chains during replication (By similarity). The nucleoprotein protein N prevents excessive phosphorylation of P, which leads to down-regulation of viral transcription/ replication. Participates, together with N, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place (By similarity)

Subunit

Homotetramer (PubMed:40268911). Interacts (via multimerization domain and XD domain) with polymerase L; this interaction forms the polymerase L-P complex (PubMed:40268911). Interacts (via N-terminus) with N0 (via Ncore); this interaction allows P to chaperon N0 to avoid N polymerization and non-specific RNA binding before encapsidation (By similarity). Interacts (via C-terminus) with N-RNA template (via Ntail); this interaction maintains the P/L complex anchored to the nucleocapsid template during the sequential transcription (PubMed:27936158). Interacts (via C-terminus) with protein C; this interaction allows C to associate with the ribonucleocapsid and regulate the polymerase activity (Probable)

Gene: P/V  |  Organism: Measles virus (strain Edmonston)
Key Publications

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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