Recombinant Measles virus Phosphoprotein (P/V), partial, Measles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus), E.coli, N-terminal 6xHis-KSI-tagged, 21.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Measles virus Phosphoprotein (P/V), partial

Recombinant Measles virus Phosphoprotein (P/V), partial — Essential cofactor of the RNA polymerase L that plays a central role in the transcription and replication by forming the polymerase complex with RNA polymerase L and recruiting L to the genomic N-R… Purity >90%.

SKU: BCRECP-003990 Categories: ,
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Product Specifications

Product SkuBCRECP-003990
Product DescriptionRecombinant Measles virus Phosphoprotein (P/V) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 459-507aa. [Accession | P03422].
Uniprot No.P03422
Gene NamesP/V
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region459-507aa
SpeciesMeasles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight21.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Essential cofactor of the RNA polymerase L that plays a central role in the transcription and replication by forming the polymerase complex with RNA polymerase L and recruiting L to the genomic N-RNA template for RNA synthesis. Also plays a central role in the encapsidation of nascent RNA chains by forming the encapsidation complex with the nucleocapsid protein N (N-P complex). Acts as a chaperone for newly synthesized free N protein, so-called N0, allowing encapsidation of nascent RNA chains during replication.

Product Specifications

Recombinant Measles virus Phosphoprotein (P/V), partial is a recombinant protein from Measles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus), expressed in E.coli, covering amino acids 459-507aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 21.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Essential cofactor of the RNA polymerase L that plays a central role in the transcription and replication by forming the polymerase complex with RNA polymerase L and recruiting L to the genomic N-RNA template for RNA synthesis (By similarity). Also plays a central role in the encapsidation of nascent RNA chains by forming the encapsidation complex with the nucleocapsid protein N (N-P complex). Acts as a chaperone for newly synthesized free N protein, so-called N0, allowing encapsidation of nascent RNA chains during replication (By similarity). The nucleoprotein protein N prevents excessive phosphorylation of P, which leads to down-regulation of viral transcription/ replication. Participates, together with N, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place (By similarity)

Subunit

Homotetramer (PubMed:40268911). Interacts (via multimerization domain and XD domain) with polymerase L; this interaction forms the polymerase L-P complex (PubMed:40268911). Interacts (via N-terminus) with N0 (via Ncore); this interaction allows P to chaperon N0 to avoid N polymerization and non-specific RNA binding before encapsidation (By similarity). Interacts (via C-terminus) with N-RNA template (via Ntail); this interaction maintains the P/L complex anchored to the nucleocapsid template during the sequential transcription (PubMed:27936158). Interacts (via C-terminus) with protein C; this interaction allows C to associate with the ribonucleocapsid and regulate the polymerase activity (Probable)

Gene: P/V  |  Organism: Measles virus (strain Edmonston)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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