Recombinant Methanosarcina barkeri Pyrrolysine--tRNA ligase (pylS), Methanosarcina barkeri (strain Fusaro / DSM 804), E.coli, N-terminal 6xHis-tagged, 51.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Methanosarcina barkeri Pyrrolysine–tRNA ligase (pylS)

Recombinant Methanosarcina barkeri Pyrrolysine–tRNA ligase (pylS) — Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Purity >85%.

SKU: BCRECP-004222 Categories: ,
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Product Specifications

Product SkuBCRECP-004222
Product DescriptionRecombinant Methanosarcina barkeri Pyrrolysine--tRNA ligase (pylS) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-419aa. [Accession | Q46E77].
Uniprot No.Q46E77
Gene NamespylS
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-419aa
SpeciesMethanosarcina barkeri (strain Fusaro / DSM 804)
Tag InfoN-terminal 6xHis-tagged
Molecular weight51.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Methanosarcina barkeri Pyrrolysine–tRNA ligase (pylS) is a recombinant protein from Methanosarcina barkeri (strain Fusaro / DSM 804), expressed in E.coli, covering amino acids 1-419aa, with N-terminal 6xHis-tagged tag, molecular weight 51.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG

Subcellular Location

Cytoplasm

Gene: pylS  |  Organism: Methanosarcina barkeri (strain Fusaro / DSM 804)  |  Synonyms: Pyrrolysine--tRNA(Pyl) ligase; Pyrrolysyl-tRNA synthetase

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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