Recombinant Methanothermus fervidus DNA-binding protein HMf-2 (hmfB), Methanothermus fervidus, Baculovirus, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 11.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Methanothermus fervidus DNA-binding protein HMf-2 (hmfB)

Recombinant Methanothermus fervidus DNA-binding protein HMf-2 (hmfB) — Binds and compacts DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Purity >90%.

SKU: BCRECP-002996 Category:
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Product Specifications

Product SkuBCRECP-002996
Product DescriptionRecombinant Methanothermus fervidus DNA-binding protein HMf-2 (hmfB) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-69aa. [Accession | P19267].
Uniprot No.P19267
Gene NameshmfB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region1-69aa
SpeciesMethanothermus fervidus
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight11.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Binds and compacts DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation in vitro.

Biological Context

Subcellular Location: Cytoplasm; Chromosome

Product Specifications

Recombinant Methanothermus fervidus DNA-binding protein HMf-2 (hmfB) is a recombinant protein from Methanothermus fervidus, expressed in Baculovirus, covering amino acids 1-69aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 11.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Binds and compacts DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils (PubMed:10704305, PubMed:2377617, PubMed:28798133, PubMed:7809089). Increases the resistance of DNA to thermal denaturation in vitro (PubMed:2377617)

Subcellular Location

Cytoplasm; Chromosome

Subunit

Homodimer and heterodimer of HmfA and HmfB (PubMed:10704305, PubMed:11021968, PubMed:2377617, PubMed:28798133, PubMed:7809089). Heterodimers may be formed primarily when cells enter stationary growth (PubMed:7809089). Dimers then assemble into higher oligomers, with the DNA wrapped around the protein core (PubMed:28798133). Higher order oligomerization of these structures can give rise to long, superhelical fibers (in vitro) (PubMed:28798133)

Gene: hmfB  |  Organism: Methanothermus fervidus  |  Synonyms: Archaeal histone B
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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