Recombinant Mouse Amyloid-beta precursor protein (App)

Function

The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis

Subcellular Location

Nucleus; Cytoplasm

Tissue Specificity

Expressed in several different brain regions including hippocampus, substantia nigra pars compacta and cerebellum (PubMed:8510506). Within the cerebellum, abundantly expressed in Purkinje cells (PubMed:8510506)

Subunit

Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif. Also interacts with GPCR-like protein BPP, APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1 (By similarity). In vitro, it binds MAPT via the MT-binding domains (By similarity). Associates with microtubules in the presence of ATP and in a kinesin-dependent manner (By similarity). Interacts, through a C-terminal domain, with GNAO1 (By similarity). Amyloid-beta protein 42 binds CHRNA7 in hippocampal neurons (By similarity). Amyloid-beta associates with HADH2 (By similarity). Interacts with ANKS1B and AGER (By similarity). Interacts with CPEB1. Interacts with ITM2B. Interacts with ITM2C. Interacts with IDE. Can form homodimers; dimerization is enhanced in the presence of Cu(2+) ions. Can form homodimers; this is promoted by heparin binding (By similarity). Amyloid-beta protein 40 interacts with S100A9 (By similarity). CTF-alpha product of APP interacts with GSAP (By similarity). Isoform APP695 interacts with SORL1 (via N-terminal ectodomain); this interaction retains APP in the trans-Golgi network and reduces processing into soluble APP-alpha and amyloid-beta peptides (PubMed:16174740, PubMed:16407538). The C99 fragment also interacts with SORL1 (PubMed:16407538). Isoform APP751 interacts with SORL1 (PubMed:16174740). Isoform APP770 interacts with SORL1 (PubMed:16174740). Interacts with PLD3 (By similarity). Interacts with VDAC1 (PubMed:25168729). Interacts with NSG1; could regulate APP processing (PubMed:21084623). Amyloid-beta protein 42 interacts with FPR2 (By similarity). Interacts with SYT7 (PubMed:30429473). Interacts (via transmembrane region) with PSEN1; the interaction is direct (By similarity). Interacts with LRRK2 (PubMed:28720718). Interacts (via cytoplasmic domain) with KIF5B (By similarity). Interacts (via C-terminus) with APBB2/FE65L1 (via C-terminus) (PubMed:18650440). Interacts (via intracellular domain) with APBB3 (By similarity)

• Yamada T. et al. Complementary DNA for the mouse homolog of the human amyloid beta protein precursor. — Biochem. Biophys. Res. Commun. (1987)
• Yamada T. et al. PubMed: CI-BOROIMFVV6SK7 (MAR-)
• de Strooper B. et al. The amyloid beta protein precursor or proteinase nexin II from mouse is closer related to its human homolog than previously reported. — Biochim. Biophys. Acta (1991)
• Kumar V.B. et al. Molecular cloning, expression, and regulation of hippocampal amyloid precursor protein of senescence accelerated mouse (SAMP8). — Biochem. Cell Biol. (2001)
• Izumi R. et al. Positive and negative regulatory elements for the expression of the Alzheimer's disease amyloid precursor-encoding gene in mouse. — Gene (1992)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Yamada T. et al. Structure and expression of the alternatively-spliced forms of mRNA for the mouse homolog of Alzheimer's disease amyloid beta protein precursor. — Biochem. Biophys. Res. Commun. (1989)
• Fukuchi K. et al. Sequence of the protease inhibitor domain of the A4 amyloid protein precursor of Mus domesticus. — Nucleic Acids Res. (1989)