Recombinant Mouse Bloom syndrome protein homolog (Blm), partial, Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal MYC-tagged, 24.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Bloom syndrome protein homolog (Blm), partial

Recombinant Mouse Bloom syndrome protein homolog (Blm), partial — ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3′-5′ direction. Purity >90%.

SKU: BCRECP-002214 Category:
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Product Specifications

Product SkuBCRECP-002214
Product DescriptionRecombinant Mouse Bloom syndrome protein homolog (Blm) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal MYC-tagged. It contains 684-859aa. [Accession | O88700].
Uniprot No.O88700
Gene NamesBlm
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region684-859aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal MYC-tagged
Molecular weight24.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. Participates in DNA replication and repair. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Highly expressed in testis 12-14 days after birth (corresponding to the pachytene phase) and at much lower levels in brain, heart, liver, lung, thymus, kidney and spleen. Expressed in bone marrow

Product Specifications

Recombinant Mouse Bloom syndrome protein homolog (Blm), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 684-859aa, with N-terminal 10xHis-tagged and C-terminal MYC-tagged tag, molecular weight 24.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9840919). Participates in DNA replication and repair (By similarity). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:9840919). Negatively regulates sister chromatid exchange (SCE) (PubMed:27010503, PubMed:9840919). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Unwinds G-quadruplex DNA. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (By similarity). Recruited by the KHDC3-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks

Subcellular Location

Nucleus

Tissue Specificity

Highly expressed in testis 12-14 days after birth (corresponding to the pachytene phase) and at much lower levels in brain, heart, liver, lung, thymus, kidney and spleen (PubMed:27010503, PubMed:9840919). Expressed in bone marrow (PubMed:27010503)

Subunit

Monomer. Homodimer (via N-terminus). Homotetramer (via N-terminus); dimer of dimers. Homohexamer (via N-terminus). Self-association negatively regulates DNA unwinding amplitude and rate. Oligomer complexes dissociate into monomer in presence of ATP. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with RMI complex. Interacts directly with RMI1 (via N-terminal region) component of RMI complex. Found in a complex, at least composed of BLM, RAD51 and SPIDR; the complex formation is mediated by SPIDR. Interacts with the KHDC3/FILIA-OOEP/FLOPED scaffold complex and TRIM25 at DNA replication forks (PubMed:29125140). Interacts with ubiquitinated FANCD2 (By similarity). Interacts with SUPV3L1 (By similarity). Interacts with TOP3A (via N-terminal region). Interacts with SPIDR (via C-terminal region); the interaction is direct and required to target BLM to sites of DNA damage

Gene: Blm  |  Organism: Mus musculus  |  Synonyms: Bloom syndrome protein homolog; DNA 3'-5' helicase BLM; RecQ helicase homolog

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