Recombinant Mouse Carnosine N-methyltransferase (Carnmt1), partial, Mus musculus (Mouse), E.coli, N-terminal 6xHis-SUMO-tagged, 54.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Mouse Carnosine N-methyltransferase (Carnmt1), partial

Recombinant Mouse Carnosine N-methyltransferase (Carnmt1), partial — Protein-histidine N-methyltransferase that specifically catalyzes the N1-methylation (pros-methylation) of histidine residues in a wide range of proteins. Purity >85%.

SKU: BCRECP-002291 Categories: ,
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Product Specifications

Product SkuBCRECP-002291
Product DescriptionRecombinant Mouse Carnosine N-methyltransferase (Carnmt1) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 50-400aa. [Accession | Q80UY1].
Uniprot No.Q80UY1
Gene NamesCarnmt1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region50-400aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight54.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Protein-histidine N-methyltransferase that specifically catalyzes the N1-methylation (pros-methylation) of histidine residues in a wide range of proteins. It primarily monomethylates proteins containing RNA-binding C3H1 zinc finger motifs, such as U2AF1, ZC3H15, ZC3H18, PPP1R10, PRR3 and RNF113A, thereby modulating mRNA splicing and turnover. Additionally, it methylates the dipeptide carnosine (beta-alanyl-L-histidine) at the N1 position to generate anserine, an abundant component of vertebrate skeletal muscle.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Nucleus

Product Specifications

Recombinant Mouse Carnosine N-methyltransferase (Carnmt1), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 50-400aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 54.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Protein-histidine N-methyltransferase that specifically catalyzes the N1-methylation (pros-methylation) of histidine residues in a wide range of proteins. It primarily monomethylates proteins containing RNA-binding C3H1 zinc finger motifs, such as U2AF1, ZC3H15, ZC3H18, PPP1R10, PRR3 and RNF113A, thereby modulating mRNA splicing and turnover (PubMed:37612136). Additionally, it methylates the dipeptide carnosine (beta-alanyl-L-histidine) at the N1 position to generate anserine, an abundant component of vertebrate skeletal muscle. Acts also on other L-histidine-containing di- and tripeptides, such as Gly-Gly-His, Gly-His, and homocarnosine (GABA-His) (By similarity)

Subcellular Location

Cytoplasm, cytosol; Nucleus

Subunit

Homodimer. Each monomer accommodates one molecule of carnosine in its active pocket, precisely anchoring the histidine imidazole ring such that only N1 is exposed and deprotonated for methylation

Gene: Carnmt1  |  Organism: Mus musculus  |  Synonyms: Carnosine N-methyltransferase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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