Recombinant Mouse Cell adhesion molecule 1 (Cadm1), partial (Active)

Function

Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner (PubMed:12202822, PubMed:12799182, PubMed:12826663). Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner (PubMed:12826663). Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo (PubMed:15811952). In mast cells, may mediate attachment to and promote communication with nerves (By similarity). CADM1, together with MITF, is essential for development and survival of mast cells in vivo (PubMed:15158462, PubMed:16605125). By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node (PubMed:19752223). Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells (PubMed:24687959). Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells (PubMed:24687959). Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (PubMed:12202822, PubMed:23209303). May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons (PubMed:15707673). May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa (PubMed:12606335, PubMed:16612000)

Subcellular Location

Cell membrane (Single-pass type I membrane protein); Synaptic cell membrane (Single-pass type I membrane protein)

Tissue Specificity

Expressed dominantly in epithelial cells but not expressed in fibroblast cells (at protein level) (PubMed:12826663). Expressed in the T-cell area of lymph nodes, specifically in CD8+ and CD4- CD8- dendritic cells (at protein level) (PubMed:19752223). Expressed in CD8+ dendritic cells in the spleen (at protein level) (PubMed:19752223). Expressed in CD103+ dendritic cells in the small intestine lamina propria and mesenteric lymph nodes (at protein level) (PubMed:24687959). Expressed in brain, lung, kidney, testis, heart, spleen and liver, but not expressed in skeletal muscle (PubMed:12202822, PubMed:12242005, PubMed:12606335)

Subunit

Homodimer (via Ig-like V-type domain) (By similarity). Interacts with FARP1 (PubMed:23209303). Interacts (via Ig-like V-type domain) with CRTAM (via Ig-like V-type domain); the interaction competes with CRTAM homodimerization and CADM1 homodimerization (PubMed:15781451, PubMed:15811952). Interacts (via C-terminus) with EPB41L3/DAL1 (By similarity). The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton (By similarity). Interacts (via C-terminus) with MPP2 (via PDZ domain) (By similarity). Interacts (via C-terminus) with MPP3 (via PDZ domain); this interaction connects CADM1 with DLG1 (By similarity). Interacts (via C-terminus) with PALS2 (via PDZ domain) (PubMed:12826663)

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• Biederer T. et al. SynCAM, a synaptic adhesion molecule that drives synapse assembly. — Science (2002)
• Fujita E. et al. RA175, which is the mouse ortholog of TSLC1, a tumor suppressor gene in human lung cancer, is a cell adhesion molecule. — Exp. Cell Res. (2003)
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• Ito A. et al. A secretion form of SgIGSF/TSLC1. (SEP-)
• Fujita E. et al. Neuron-specific isoforms of RA175/TSLC1/SynCAM. (JUL-)
• Wakayama T. et al. Expression and functional characterization of the adhesion molecule spermatogenic immunoglobulin superfamily in the mouse testis. — Biol. Reprod. (2003)