Recombinant Mouse Collagen alpha-1 (IV) chain (Col4a1), partial, Mus musculus (Mouse), E.coli, C-terminal 6xHis-tagged, 31.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Collagen alpha-1 (IV) chain (Col4a1), partial

Recombinant Mouse Collagen alpha-1 (IV) chain (Col4a1), partial — Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. Purity >85%.

SKU: BCRECP-002408 Category:
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Product Specifications

Product SkuBCRECP-002408
Product DescriptionRecombinant Mouse Collagen alpha-1 (IV) chain (Col4a1) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1445-1669aa. [Accession | P02463].
Uniprot No.P02463
Gene NamesCol4a1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1445-1669aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight31.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.

Biological Context

Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Specificity: Detected in the basement membrane of the cornea (at protein level)

Product Specifications

Recombinant Mouse Collagen alpha-1 (IV) chain (Col4a1), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1445-1669aa, with C-terminal 6xHis-tagged tag, molecular weight 31.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane

Tissue Specificity

Detected in the basement membrane of the cornea (at protein level)

Subunit

There are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. Interacts with EFEMP2 (PubMed:17324935)

Gene: Col4a1  |  Organism: Mus musculus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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