Recombinant Mouse Collagen alpha-1 (XII) chain (Col12a1), partial, Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 23.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Collagen alpha-1 (XII) chain (Col12a1), partial

Recombinant Mouse Collagen alpha-1 (XII) chain (Col12a1), partial — recombinant protein from Mus musculus (Mouse), expressed in E.coli. Purity >90%.

SKU: BCRECP-002407 Category:
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Product Specifications

Product SkuBCRECP-002407
Product DescriptionRecombinant Mouse Collagen alpha-1 (XII) chain (Col12a1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 140-316aa. [Accession | Q60847].
Uniprot No.Q60847
Gene NamesCol12a1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region140-316aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight23.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.

Biological Context

Subcellular Location: Secreted, extracellular space, extracellular matrix
Tissue Specificity: Highest expression in tendons, perichondrium, skin, cornea, sclera, blood vessels, and periosteum

Product Specifications

Recombinant Mouse Collagen alpha-1 (XII) chain (Col12a1), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 140-316aa, with N-terminal 6xHis-tagged tag, molecular weight 23.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix

Subcellular Location

Secreted, extracellular space, extracellular matrix

Tissue Specificity

Highest expression in tendons, perichondrium, skin, cornea, sclera, blood vessels, and periosteum

Subunit

Trimer of identical chains each containing 190 kDa of non-triple-helical sequences

Gene: Col12a1  |  Organism: Mus musculus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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