Recombinant Mouse Complement C1s-B subcomponent (C1sb), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 82.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Complement C1s-B subcomponent (C1sb)

Recombinant Mouse Complement C1s-B subcomponent (C1sb) — Serine protease component of the complement C1 complex, which catalyzes cleavage and activation of C2 and C4, the next components of the classical complement pathway. Purity >85%.

SKU: BCRECP-002270 Category:
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Product Specifications

Product SkuBCRECP-002270
Product DescriptionRecombinant Mouse Complement C1s-B subcomponent (C1sb) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 16-688aa. [Accession | Q8CFG8].
Uniprot No.Q8CFG8
Gene NamesC1sb
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region16-688aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight82.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Serine protease component of the complement C1 complex, which catalyzes cleavage and activation of C2 and C4, the next components of the classical complement pathway. Also cleaves IGFBP5 and thereby inhibits the trophic effects of IGF1.

Biological Context

Subcellular Location: Secreted; Cell surface
Tissue Specificity: Specifically expressed in male reproductive tissues

Product Specifications

Recombinant Mouse Complement C1s-B subcomponent (C1sb) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 16-688aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 82.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Serine protease component of the complement C1 complex, which catalyzes cleavage and activation of C2 and C4, the next components of the classical complement pathway. Also cleaves IGFBP5 and thereby inhibits the trophic effects of IGF1

Subcellular Location

Secreted; Cell surface

Tissue Specificity

Specifically expressed in male reproductive tissues

Subunit

Core component of the complement C1 complex, a calcium-dependent complex composed of 1 molecule of the C1Q subcomplex, 2 molecules of C1R and 2 molecules of C1S. The C1Q subcomplex is composed 18 subunits: 3 chains of C1QA, C1QB, and C1QC trimerize to form 6 collagen-like triple helices connected to six globular ligand-recognition modules

Gene: C1s2  |  Organism: Mus musculus  |  Synonyms: C1 esterase; Complement C1s-B subcomponent; Complement component 1 subcomponent s-B; Complement component 1, S subcomponent 2

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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