Recombinant Mouse Ephrin-A1 (Efna1), partial (Active), Mus musculus (Mouse), Mammalian cell, C-terminal 6xHis-hFc-tagged, 47.3 kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Ephrin-A1 (Efna1), partial (Active)

Recombinant Mouse Ephrin-A1 (Efna1), partial (Active) — Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Purity >90%.

SKU: BCACP-001110 Category:
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Product SKUBCACP-001110
Product DescriptionRecombinant Mouse Ephrin-A1 (Efna1) protein is expressed from Mammalian cell with C-terminal 6xHis-hFc-tagged. It contains 19-182aa. [Accession | P52793].
Uniprot No.P52793
Gene NamesEfna1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region19-182aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-hFc-tagged
Molecular weight47.3 kDa
ActivityMeasured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤2.0 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesEfna1; Epgl1; Epl1; Lerk1; Ephrin-A1; EPH-related receptor tyrosine kinase ligand 1; LERK-1; Immediate early response protein B61) [Cleaved into: Ephrin-A1; secreted form]

Function

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Involved in angiogenesis, regulating vascular endothelial cell differentiation and migration through activation of EPHA2.

Product Specifications

Recombinant Mouse Ephrin-A1 (Efna1), partial (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 19-182aa, with C-terminal 6xHis-hFc-tagged tag, molecular weight 47.3 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells (PubMed:16782872, PubMed:18387945, PubMed:20960543). Involved in angiogenesis, regulating vascular endothelial cell differentiation and migration through activation of EPHA2 (PubMed:16782872). Acts as a ligand for EPHA3, inhibiting epithelial-to-mesenchymal transition of cardiac cells and playing a role in heart development (PubMed:20960543). May also contribute to dendritic spine morphogenesis (PubMed:17143272)

Gene: Efna1  |  Organism: Mus musculus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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