Recombinant Mouse Erythroferrone (Erfe), Mus musculus (Mouse), E.coli, N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged, 53.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Erythroferrone (Erfe)

Recombinant Mouse Erythroferrone (Erfe) — Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, the… Purity >85%.

SKU: BCRECP-002655 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-002655
Product DescriptionRecombinant Mouse Erythroferrone (Erfe) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 25-340aa. [Accession | Q6PGN1].
Uniprot No.Q6PGN1
Gene NamesErfe
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region25-340aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Molecular weight53.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.

Product Specifications

Recombinant Mouse Erythroferrone (Erfe) — Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, the… Purity >85%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores (PubMed:24880340, PubMed:30097509, PubMed:31800957, PubMed:34002695). Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake (PubMed:22351773). Inhibits apoptosis and inflammatory response in cardiomyocytes via promotion of sphingosine-1-phosphate (S1P) and cAMP-dependent activation of AKT signaling (PubMed:30566056). Inhibits autophagy induced by nutrient deficiency in hepatocytes via promoting the phosphorylation of IRS1, AKT, and MTOR, and thereby subsequent activation of the AKT-MTOR signaling pathway (PubMed:24187137). Negatively regulates the differentiation of osteoblasts, potentially via sequestering BMP2, and thereby inhibits the activation of SMAD signaling (PubMed:34002695). The reduction in BMP2 signaling in osteoblasts also results in an increase in expression of the osteoclastogenesis-promoting factors TNFSF11/RANKL and SOST, thereby indirectly promotes bone resorption (PubMed:34002695)

Subcellular Location

Secreted

Tissue Specificity

Expressed in the soleus muscle in the leg (at protein level) (PubMed:22351773, PubMed:30566056). Found in blood (at protein level) (PubMed:22351773, PubMed:24187137). Weakly expressed in the heart (at protein level) (PubMed:22351773, PubMed:30566056). Predominantly expressed in skeletal muscle and, at much lower levels, in other tissues, including lung, eye, smooth muscle, brain and kidney (PubMed:22351773, PubMed:24187137). Within skeletal muscles, higher expression levels in soleus as compared with plantaris (PubMed:22351773). Expressed in osteoblasts, mature osteoclasts and erythroblasts (PubMed:34002695). When fasting, females tend to have higher circulating levels than males (PubMed:22351773). Obese mice tend to have lower expression and circulating levels as compared to lean animals (PubMed:22351773). Following EPO treatment, only expressed in bone marrow and spleen (PubMed:24880340)

Subunit

Homodimer; disulfide-linked (PubMed:22351773). Forms trimer, hexamers and higher molecular weight oligomers (PubMed:32602701). May form heteromeric complexes with C1QTNF2 and C1QTNF12 and, to a lesser extent, with C1QTNF5 and C1QTNF10 (PubMed:22351773). Interacts with BMP5 and BMP7; the interaction inhibits BMP-induced transcription of HAMP (PubMed:30097509). Interacts with BMP6; the interaction inhibits BMP-induced transcription of HAMP (PubMed:30097509, PubMed:31800957). Interacts with BMP2 (PubMed:31800957). Interacts with heterodimers composed of BMP2 and BMP6 in vitro, the interaction inhibits the heterodimer binding to its receptor BMPR1A /ALK3 and thereby suppresses expression of HAMP (PubMed:31800957)

Gene: Erfe  |  Organism: Mus musculus  |  Synonyms: Complement C1q tumor necrosis factor-related protein 15; Myonectin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top