Recombinant Mouse F-box only protein 32 (Fbxo32)

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. Negatively regulates macrophage efferocytosis by promoting the ubiquitination of the transcription factor KLF4, suppressing the receptor tyrosine kinase MERTK transcription (PubMed:39986490). Regulates LPS-induced apoptosis and mitochondrial dysfunction through ANXA1 ubiquitination and degradation (PubMed:24789905). Mediates cyclin D1 protein stabilization via 'Lys-27'-linked ubiquitination (By similarity)

Subcellular Location

Cytoplasm; Nucleus

Tissue Specificity

Specifically expressed in cardiac and skeletal muscle

Subunit

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32. Interacts with KLF4; this interaction promotes KLF4 ubiquitination and degradation (PubMed:39986490). Negatively regulates macrophage efferocytosis by promoting the ubiquitination of the transcription factor KLF4, suppressing the receptor tyrosine kinase MERTK transcription (PubMed:39986490)

Pathway

Protein modification; protein ubiquitination

• Gomes M.D. et al. Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy. — Proc. Natl. Acad. Sci. U.S.A. (2001)
• Carninci P. et al. The transcriptional landscape of the mammalian genome. — Science (2005)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Zaglia T. et al. Atrogin-1 deficiency promotes cardiomyopathy and premature death via impaired autophagy. — J. Clin. Invest. (2014)
• Zhu H. et al. Methylglyoxal deteriorates macrophage efferocytosis in diabetic wounds through ROS-induced ubiquitination degradation of KLF4. — Free Radic. Biol. Med. (2025)