Recombinant Mouse Fibroblast growth factor 1 (Fgf1) (Active), Mus musculus (Mouse), E.coli, Tag-Free, 15.7 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Mouse Fibroblast growth factor 1 (Fgf1) (Active)

Recombinant Mouse Fibroblast growth factor 1 (Fgf1) (Active) — Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Purity >95%.

SKU: BCACP-001232 Categories: ,
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Product SKUBCACP-001232
Product DescriptionRecombinant Mouse Fibroblast growth factor 1 (Fgf1) protein is expressed from E.coli with Tag-Free. It contains 16-155aa. [Accession | P61148].
Uniprot No.P61148
Gene NamesFgf1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region16-155aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight15.7 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesFgf1; Fgf-1; Fgfa; Fibroblast growth factor 1; FGF-1; Acidic fibroblast growth factor; aFGF; Heparin-binding growth factor 1; HBGF-1

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins.

Product Specifications

Recombinant Mouse Fibroblast growth factor 1 (Fgf1) (Active) is a recombinant protein, expressed in E.coli, covering amino acids 16-155aa, with Tag-Free tag, molecular weight 15.7 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis

Gene: Fgf1  |  Organism: Mus musculus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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