Recombinant Mouse fMet-Leu-Phe receptor (Fpr1), partial, Mus musculus (Mouse), E.coli, N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged, 33.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse fMet-Leu-Phe receptor (Fpr1), partial

Recombinant Mouse fMet-Leu-Phe receptor (Fpr1), partial — (Microbial infection) Used by Y. Purity >85%.

SKU: BCRECP-002781 Category:
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Product Specifications

Product SkuBCRECP-002781
Product DescriptionRecombinant Mouse fMet-Leu-Phe receptor (Fpr1) Protein is expressed from E.coli with N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged. It contains 1-35aa. [Accession | P33766].
Uniprot No.P33766
Gene NamesFpr1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-35aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-GST-tagged and C-terminal Myc-tagged
Molecular weight33.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

(Microbial infection) Used by Y. pestis as a receptor on immune cells. Upon infection, Y.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed in neutrophils, dendritic cells, microglia, spleen, lung and liver. Low level of expression in the vomeronasal organ

Product Specifications

Recombinant Mouse fMet-Leu-Phe receptor (Fpr1), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-35aa, with N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged tag, molecular weight 33.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

(Microbial infection) Used by Y. pestis as a receptor on immune cells. Upon infection, Y. pestis releases N-formyl peptides that activate FPR1-mediated immune signaling and chemotaxis. This leads to Y. pestis docking on FPR1 via the lcrV needle cap protein of its type III secretion system (T3SS) followed by the delivery of effector proteins into host immune cells, ultimately triggering immune cell apoptosis

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Tissue Specificity

Expressed in neutrophils, dendritic cells, microglia, spleen, lung and liver. Low level of expression in the vomeronasal organ

Subunit

Homodimer. Heterodimer with FPR2 or FPR3; the heterodimerization with FPR2 is potentiated in response to anti-inflammatory agonists such as lipoxin A4 and ANXA1. Forms a complex with heterotrimeric G(i) protein composed of GNAI1, GNB1 and GNG2 subunits

Gene: Fpr1  |  Organism: Mus musculus  |  Synonyms: N-formylpeptide chemoattractant receptor; fMet-Leu-Phe receptor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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