Recombinant Mouse Gasdermin-D (Gsdmd), partial, Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 30.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Gasdermin-D (Gsdmd), partial

Recombinant Mouse Gasdermin-D (Gsdmd), partial — Produced by the cleavage by papain allergen. Purity >90%.

SKU: BCRECP-002928 Category:
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Product Specifications

Product SkuBCRECP-002928
Product DescriptionRecombinant Mouse Gasdermin-D (Gsdmd) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 277-487aa. [Accession | Q9D8T2].
Uniprot No.Q9D8T2
Gene NamesGsdmdc1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region277-487aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight30.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Produced by the cleavage by papain allergen. After cleavage, moves to the plasma membrane and homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the specific release of mature interleukin-33 (IL33), promoting type 2 inflammatory immune response.

Biological Context

Subcellular Location: Cytoplasm, cytosol
Tissue Specificity: Highly expressed in brain endothelial cells

Product Specifications

Recombinant Mouse Gasdermin-D (Gsdmd), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 277-487aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 30.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Produced by the cleavage by papain allergen (PubMed:35794369). After cleavage, moves to the plasma membrane and homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the specific release of mature interleukin-33 (IL33), promoting type 2 inflammatory immune response (PubMed:35749514, PubMed:35794369)

Subcellular Location

Cytoplasm, cytosol

Tissue Specificity

Highly expressed in brain endothelial cells

Subunit

Homooligomer; homooligomeric ring-shaped pore complex containing 27-28 subunits when inserted in the membrane (PubMed:34289345). Homooligomerization is promoted by the mTORC1 complex in macrophages (PubMed:34289345). In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor (PubMed:26611636). Although this recruitment is also observed in the absence of PYCARD, it is more efficient in its presence (PubMed:26611636)

Gene: Gsdmd  |  Organism: Mus musculus  |  Synonyms: Gasdermin domain-containing protein 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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